Typical Testing Data/Standard Curve (for reference only)
Human V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN) ELISA Kit | LYN elisa kit
Human V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN) ELISA Kit
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to LYN. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to LYN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LYN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LYN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a tyrosine protein kinase, which maybe involved in the regulation of mast cell degranulation, and erythroid differentiation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]
Uniprot Description
LYN: a tyrosine kinase of the Src family that plays a role in B cell antigen-receptor signaling.
Protein type: Protein kinase, TK; Kinase, protein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Oncoprotein; TK group; Src family
Chromosomal Location of Human Ortholog: 8q13
Cellular Component: Golgi apparatus; mitochondrial crista; extrinsic to internal side of plasma membrane; mast cell granule; perinuclear region of cytoplasm; postsynaptic density; cytoplasm; plasma membrane; mitochondrial intermembrane space; cytosol; nucleus; lipid raft
Molecular Function: integrin binding; protein binding; enzyme binding; ubiquitin protein ligase binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; platelet-derived growth factor receptor binding; phosphoprotein binding; receptor signaling protein tyrosine kinase activity; glycosphingolipid binding; SH3 domain binding; ATP binding; receptor binding
Biological Process: regulation of mast cell degranulation; negative regulation of MAP kinase activity; central nervous system development; viral reproduction; response to toxin; positive regulation of B cell receptor signaling pathway; protein amino acid phosphorylation; response to organic cyclic substance; response to carbohydrate stimulus; regulation of B cell apoptosis; B cell receptor signaling pathway; erythrocyte differentiation; oligodendrocyte development; tolerance induction to self antigen; response to drug; platelet activation; negative regulation of immune response; response to sterol depletion; immune response-regulating cell surface receptor signaling pathway; response to amino acid stimulus; regulation of erythrocyte differentiation; histamine secretion by mast cell; T cell costimulation; leukocyte migration; cytokine secretion; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of mast cell activation; axon guidance; regulation of cytokine secretion; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; response to hormone stimulus; negative regulation of B cell proliferation; signal transduction; response to insulin stimulus; regulation of cytokine production; negative regulation of cell proliferation; regulation of cell adhesion mediated by integrin; platelet degranulation; B cell homeostasis; negative regulation of protein amino acid phosphorylation; positive regulation of cell proliferation; ephrin receptor signaling pathway; lipopolysaccharide-mediated signaling pathway; Fc receptor mediated inhibitory signaling pathway; Fc receptor mediated stimulatory signaling pathway; response to axon injury; inflammatory response; regulation of B cell receptor signaling pathway; regulation of protein amino acid phosphorylation; negative regulation of myeloid leukocyte differentiation; negative regulation of toll-like receptor 2 signaling pathway; positive regulation of phosphoinositide 3-kinase activity; positive regulation of cell motility; regulation of release of sequestered calcium ion into cytosol; positive regulation of tyrosine phosphorylation of STAT protein; negative regulation of toll-like receptor 4 signaling pathway; regulation of inflammatory response; cellular response to extracellular stimulus; innate immune response; blood coagulation; response to DNA damage stimulus; positive regulation of cell migration
Research Articles on LYN
Similar Products
Product Notes
The Human LYN lyn (Catalog #AAA2023647) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2023647 ELISA Kit recognizes Human LYN. It is sometimes possible for the material contained within the vial of "V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
