Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Urocanate hydratase (UROC1) Standard Curve.)
Human Urocanate Hydratase (UROC1) ELISA Kit | UROC1 elisa kit
Human Urocanate Hydratase (UROC1) ELISA Kit
Gene Names
UROC1; UROCD; HMFN0320
Reactivity
Human
Synonyms
Urocanate Hydratase (UROC1); Human Urocanate Hydratase (UROC1) ELISA Kit; UROC1; FLJ31300; HMFN0320; MGC135007; MGC135008; imidazolonepropionate hydrolase; UROC1 elisa kit
Reactivity
Human
Specificity
Human Urocanate hydratase (UROC1) ELISA Kit has high sensitivity and excellent specificity for detection of Human UROC1. No significant cross-reactivity or interference between Human UROC1 and analogues was observed.
Sequence Length
736
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Urocanate hydratase (UROC1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Urocanate hydratase (UROC1) Standard Curve.)
Related Product Information for UROC1 elisa kit
Description: This Human Urocanate hydratase (UROC1) ELISA Kit employs a two-site sandwich ELISA to quantitate UROC1 in samples. An antibody specific for UROC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UROC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UROC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UROC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Catabolism of histidine begins with the conversion of histidine to urocanic acid by histidase (HAL). UROC1, or urocanase (EC 4.2.1.49), catalyzes the second step in histidine catabolism, the metabolism of urocanic acid to formiminoglutamic acid. Espinos et al. (2009) cloned human UROC1. The deduced protein contains a C-terminal catalytic domain with an evolutionarily conserved catalytic arginine (arg450). UROC1 had an apparent molecular mass of 74.8 kD by SDS-PAGE.Using a spectrophotometric assay, Espinos et al. (2009) demonstrated that purified recombinant UROC1 had robust urocanase activity.Espinos et al. (2009) determined that the UROC1 gene contains 20 exons.
Background: Catabolism of histidine begins with the conversion of histidine to urocanic acid by histidase (HAL). UROC1, or urocanase (EC 4.2.1.49), catalyzes the second step in histidine catabolism, the metabolism of urocanic acid to formiminoglutamic acid. Espinos et al. (2009) cloned human UROC1. The deduced protein contains a C-terminal catalytic domain with an evolutionarily conserved catalytic arginine (arg450). UROC1 had an apparent molecular mass of 74.8 kD by SDS-PAGE.Using a spectrophotometric assay, Espinos et al. (2009) demonstrated that purified recombinant UROC1 had robust urocanase activity.Espinos et al. (2009) determined that the UROC1 gene contains 20 exons.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
urocanate hydratase isoform 2
NCBI Official Synonym Full Names
urocanate hydratase 1
NCBI Official Symbol
UROC1
NCBI Official Synonym Symbols
UROCD; HMFN0320
NCBI Protein Information
urocanate hydratase
UniProt Protein Name
Urocanate hydratase
UniProt Gene Name
UROC1
UniProt Synonym Gene Names
Urocanase
NCBI Description
This gene encodes an enzyme involved in histidine catabolism, metabolizing urocanic acid to formiminoglutamic acid. The gene product is known to protect the skin from ultra violet rays and is contained in human sweat. Deficiency of this gene product in the liver is an apparent cause of cognitive disability. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
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Product Notes
The Human UROC1 uroc1 (Catalog #AAA9713751) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713751 ELISA Kit recognizes Human UROC1. It is sometimes possible for the material contained within the vial of "Urocanate Hydratase (UROC1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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