Human Uridine Cytidine Kinase 2 (UCK2) ELISA Kit | UCK2 elisa kit

Human Uridine Cytidine Kinase 2 (UCK2) ELISA Kit

Cat. Num. AAA2707589

• Gene Names: UCK2; UK; UMPK; TSA903
• Reactivity: Human
• Synonyms: Uridine Cytidine Kinase 2 (UCK2); Human Uridine Cytidine Kinase 2 (UCK2) ELISA Kit; UK; UMPK; Uridine Monophosphate Kinase; Cytidine monophosphokinase 2; Testis-specific protein TSA903; Uridine monophosphokinase 2; UCK2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Uridine Cytidine Kinase 2 (UCK2).; No significant cross-reactivity or interference between Uridine Cytidine Kinase 2 (UCK2) and analogues was observed.
• Sequence Length: 261

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.056ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Uridine Cytidine Kinase 2 (UCK2) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Uridine Cytidine Kinase 2 (UCK2) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for UCK2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uridine Cytidine Kinase 2 (UCK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Uridine Cytidine Kinase 2 (UCK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Uridine Cytidine Kinase 2 (UCK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Uridine Cytidine Kinase 2 (UCK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 18699734
• NCBI GeneID: 7371
• NCBI Accession #: NP_036606.2
• NCBI GenBank Nucleotide #: NP_036606.2
• UniProt Accession #: Q9BZX2
• NCBI Official Full Name: uridine-cytidine kinase 2 isoform 1
• NCBI Official Synonym Full Names: uridine-cytidine kinase 2
• NCBI Official Symbol: UCK2
• NCBI Official Synonym Symbols: UK; UMPK; TSA903
• NCBI Protein Information: uridine-cytidine kinase 2
• UniProt Protein Name: Uridine-cytidine kinase 2
• Protein Family: Uridine-cytidine kinase
• UniProt Gene Name: UCK2
• UniProt Synonym Gene Names: UMPK; UCK 2
• UniProt Entry Name: UCK2_HUMAN

NCBI Description

This gene encodes a pyrimidine ribonucleoside kinase. The encoded protein (EC 2.7.1.48) catalyzes phosphorylation of uridine and cytidine to uridine monophosphate (UMP) and cytidine monophosphate (CMP), respectively.[provided by RefSeq, Oct 2010]

Uniprot Description

UCK2: Phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. Does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Can use ATP or GTP as a phosphate donor. Can also phosphorylate cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4- thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)- benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5- methylcytidine, and N(4)-anisoylcytidine. Belongs to the uridine kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.1.48; Nucleotide Metabolism - pyrimidine; Kinase, other; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: intracellular membrane-bound organelle; cytosol

Molecular Function: nucleoside kinase activity; uridine kinase activity; ATP binding

Biological Process: pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; CMP salvage; pyrimidine nucleoside salvage; feeding behavior; response to axon injury; phosphorylation