Rabbit HisRS Polyclonal Antibody | anti-HisRS antibody

HisRS (E492) Polyclonal Antibody

Cat. Num. AAA3001887

• Gene Names: HARS1; HRS; HARS; CMT2W; USH3B
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: HisRS; Polyclonal Antibody; HisRS (E492) Polyclonal Antibody; Histidyl-tRNA synthetase; cytoplasmic; Histidine--tRNA ligase; HARS; HRS; anti-HisRS antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: HisRS (E492) polyclonal antibody detects endogenous levels of HisRS protein
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 469

• Applicable Applications for anti-HisRS antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 361-410 of Human HisRS.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of HisRS (E492) pAb in paraffin-embedded human brain carcinoma tissue at 1:50.)

Related Product Information for anti-HisRS antibody

Histidyl-tRNA synthetase (HisRS, Jo-1) is an aminoacyl-tRNA synthetase that charges transfer RNAs (tRNAs) with a cognate histidine residue to produce histidyl-tRNA. Histidyl-tRNA is essential for the incorporation of histidine into proteins. Systemic autoimmune diseases lead to aminoacyl-tRNA synthetase autoantibodies that are directed against self structures. HisRS is an antigen in systemic autoimmune diseases such as rheumatic arthritis or polymyositis with associated interstitial lung disease, thrombocytopenia and Raynauds phenomenon (vasospastic attacks that cause blood vessels to constrict). HisRS autoantibodies are unlike anti-synthetase antibodies because they bind the nonlinear, exposed epitopes on native HisRS when the enzyme is complexed to tRNA(His).

NCBI and Uniprot Product Information

• NCBI GI #: 384475552
• NCBI GeneID: 3035
• NCBI Accession #: NP_001244969.1
• NCBI GenBank Nucleotide #: NP_001244969.1
• UniProt Accession #: P12081
• Molecular Weight: ~ 60kDa
• NCBI Official Full Name: histidine--tRNA ligase, cytoplasmic isoform 2
• NCBI Official Synonym Full Names: histidyl-tRNA synthetase 1
• NCBI Official Symbol: HARS1
• NCBI Official Synonym Symbols: HRS; HARS; CMT2W; USH3B
• NCBI Protein Information: histidine--tRNA ligase, cytoplasmic
• UniProt Protein Name: Histidine--tRNA ligase, cytoplasmic
• UniProt Gene Name: HARS
• UniProt Synonym Gene Names: HRS; HisRS
• UniProt Entry Name: SYHC_HUMAN

NCBI Description

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

HARS: Defects in HARS are a cause of Usher syndrome type 3B (USH3B). USH3B is a syndrome characterized by progressive vision and hearing loss during early childhood. Some patients have the so-called 'Charles Bonnet syndrome,' involving decreased visual acuity and vivid visual hallucinations. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa with sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH3 is characterized by postlingual, progressive hearing loss, variable vestibular dysfunction, and onset of retinitis pigmentosa symptoms, including nyctalopia, constriction of the visual fields, and loss of central visual acuity, usually by the second decade of life. Belongs to the class-II aminoacyl-tRNA synthetase family.

Protein type: Translation; EC 6.1.1.21; Ligase

Chromosomal Location of Human Ortholog: 5q31.3

Cellular Component: cytoplasm; cytosol

Molecular Function: histidine-tRNA ligase activity; ATP binding

Biological Process: tRNA aminoacylation for protein translation; translation; gene expression; histidyl-tRNA aminoacylation

Disease: Usher Syndrome, Type Iiib