Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ubiquitin-like modifier-activating enzyme ATG7 (ATG7) Standard Curve.)
Human Ubiquitin-Like Modifier-Activating Enzyme ATG7 (ATG7) ELISA Kit | ATG7 elisa kit
Human Ubiquitin-Like Modifier-Activating Enzyme ATG7 (ATG7) ELISA Kit
Gene Names
ATG7; GSA7; APG7L; APG7-LIKE
Reactivity
Human
Synonyms
Ubiquitin-Like Modifier-Activating Enzyme ATG7 (ATG7); Human Ubiquitin-Like Modifier-Activating Enzyme ATG7 (ATG7) ELISA Kit; ATG7; APG7-LIKE; APG7L; DKFZp434N0735; GSA7; APG7 autophagy 7-like; ubiquitin activating enzyme E1-like protein; ATG7 elisa kit
Reactivity
Human
Specificity
Human Ubiquitin-like modifier-activating enzyme ATG7 (ATG7) ELISA Kit has high sensitivity and excellent specificity for detection of Human ATG7. No significant cross-reactivity or interference between Human ATG7 and analogues was observed.
Sequence Length
676
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ubiquitin-like modifier-activating enzyme ATG7 (ATG7) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Ubiquitin-like modifier-activating enzyme ATG7 (ATG7) Standard Curve.)
Related Product Information for ATG7 elisa kit
Description: This Human Ubiquitin-like modifier-activating enzyme ATG7 (ATG7) ELISA Kit employs a two-site sandwich ELISA to quantitate ATG7 in samples. An antibody specific for ATG7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ATG7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ATG7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ATG7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: ATG7 was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vaculuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. The deduced 703-amino acid protein contains a central putative E1-like ATP-binding site (GxGxxG), conserved charged amino acids flanking the GxGxxG motif, and a putative E1 active site with a conserved catalytic cysteine. APG7L shares similarity with the E1 enzymes UBA2 and UBA3 (UBE1C), and it shares 38% identity with yeast Apg7. EST database analysis indicated that APG7L is expressed by many diverse tissues.
Background: ATG7 was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vaculuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. The deduced 703-amino acid protein contains a central putative E1-like ATP-binding site (GxGxxG), conserved charged amino acids flanking the GxGxxG motif, and a putative E1 active site with a conserved catalytic cysteine. APG7L shares similarity with the E1 enzymes UBA2 and UBA3 (UBE1C), and it shares 38% identity with yeast Apg7. EST database analysis indicated that APG7L is expressed by many diverse tissues.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ubiquitin-like modifier-activating enzyme ATG7 isoform b
NCBI Official Synonym Full Names
autophagy related 7
NCBI Official Symbol
ATG7
NCBI Official Synonym Symbols
GSA7; APG7L; APG7-LIKE
NCBI Protein Information
ubiquitin-like modifier-activating enzyme ATG7
UniProt Protein Name
Ubiquitin-like modifier-activating enzyme ATG7
Protein Family
UniProt Gene Name
ATG7
UniProt Synonym Gene Names
APG7L; APG7-like; hAGP7
UniProt Entry Name
ATG7_HUMAN
NCBI Description
This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Research Articles on ATG7
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Product Notes
The Human ATG7 atg7 (Catalog #AAA9715961) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715961 ELISA Kit recognizes Human ATG7. It is sometimes possible for the material contained within the vial of "Ubiquitin-Like Modifier-Activating Enzyme ATG7 (ATG7), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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