Human Ubiquitin like modifier activating enzyme ATG7 (ATG7) ELISA Kit | ATG7 elisa kit

Human Ubiquitin like modifier activating enzyme ATG7 (ATG7) ELISA Kit

Cat. Num. AAA7208439

• Gene Names: ATG7; GSA7; APG7L; APG7-LIKE
• Reactivity: Human
• Synonyms: Ubiquitin like modifier activating enzyme ATG7 (ATG7); Human Ubiquitin like modifier activating enzyme ATG7 (ATG7) ELISA Kit; ATG7 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 100-2500pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This ATG7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATG7. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATG7 elisa kit

Principle of the assay: ATG7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATG7 antibody and an ATG7-HRP conjugate. The assay sample and buffer are incubated together with ATG7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATG7 concentration since ATG7 from samples and ATG7-HRP conjugate compete for the anti-ATG7 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATG7 from the sample, fewer sites are left to bind ATG7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATG7 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 62286592
• NCBI GeneID: 10533
• Molecular Weight: 68,618 Da
• NCBI Official Full Name: Ubiquitin-like modifier-activating enzyme ATG7
• NCBI Official Synonym Full Names: autophagy related 7
• NCBI Official Symbol: ATG7
• NCBI Official Synonym Symbols: GSA7; APG7L; APG7-LIKE
• NCBI Protein Information: ubiquitin-like modifier-activating enzyme ATG7; hAGP7; autophagy-related protein 7; ATG12-activating enzyme E1 ATG7; ATG7 autophagy related 7 homolog; ubiquitin activating enzyme E1-like protein; ubiquitin-activating enzyme E1-like protein
• UniProt Protein Name: Ubiquitin-like modifier-activating enzyme ATG7
• Protein Family: Ubiquitin-like modifier-activating enzyme
• UniProt Gene Name: ATG7
• UniProt Synonym Gene Names: APG7L; APG7-like; hAGP7
• UniProt Entry Name: ATG7_HUMAN

NCBI Description

This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. [provided by RefSeq, Jan 2009]

Uniprot Description

ATG7: Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3. Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. Belongs to the ATG7 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3p25.3

Cellular Component: cytoplasm; axoneme; cytosol

Molecular Function: protein binding; protein homodimerization activity; APG8 activating enzyme activity; APG12 activating enzyme activity; ubiquitin activating enzyme activity; transcription factor binding

Biological Process: positive regulation of apoptosis; regulation of protein ubiquitination; protein ubiquitination; pyramidal neuron development; adult walking behavior; post-embryonic development; protein transport; cardiac muscle cell development; protein amino acid lipidation; protein catabolic process; positive regulation of autophagy; amino acid metabolic process; central nervous system neuron axonogenesis; cerebellar Purkinje cell layer development; protein modification process; C-terminal protein lipidation; cellular response to starvation; liver development; neurological system process; cellular response to nitrogen starvation; protein modification by small protein conjugation; mitochondrion degradation; positive regulation of protein catabolic process; positive regulation of protein modification process; cerebral cortex development; positive regulation of macroautophagy; negative regulation of apoptosis