Human Ubiquitin D (UBD) ELISA Kit | UBD elisa kit

Human Ubiquitin D (UBD) ELISA Kit

Cat. Num. AAA9713771

• Gene Names: UBD; FAT10; UBD-3; GABBR1
• Reactivity: Human
• Synonyms: Ubiquitin D (UBD); Human Ubiquitin D (UBD) ELISA Kit; UBD; DADB-115L1.4; FAT10; GABBR1; UBD-3; diubiquitin; UBD elisa kit

• Reactivity: Human
• Specificity: Human Ubiquitin D (UBD) ELISA Kit has high sensitivity and excellent specificity for detection of Human UBD. No significant cross-reactivity or interference between Human UBD and analogues was observed.
• Sequence Length: 165

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Ubiquitin D (UBD) Standard Curve.)

Related Product Information for UBD elisa kit

Description: This Human Ubiquitin D (UBD) ELISA Kit employs a two-site sandwich ELISA to quantitate UBD in samples. An antibody specific for UBD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UBD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBD bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Ubiquitin D is a protein encoded by the UBD gene.Using selective cDNA hybridization of a YAC containing the HLA-F locus region on chromosome 6, followed by Southern and Northern blot analyses, Fan et al. (1996) isolated a cDNA, which they called 1F12, encoding a protein similar to diubiquitin. The 1.1-kb transcript was detected only in B-cell lines transformed by Epstein-Barr virus. By subtractive hybridization of dendritic cell (DC) libraries and EST database searching, Bates et al. (1997) obtained a full-length cDNA encoding diubiquitin. The predicted 165-amino acid protein has 2 ubiquitin-like domains, each of which has 2 cysteines. Southern blot analysis indicated that the cDNA represents a single-copy gene.

NCBI and Uniprot Product Information

• NCBI GI #: 222352096
• NCBI GeneID: 10537
• NCBI Accession #: NP_006389.2
• NCBI GenBank Nucleotide #: NP_006389.2
• UniProt Accession #: O15205
• NCBI Official Full Name: ubiquitin D
• NCBI Official Synonym Full Names: ubiquitin D
• NCBI Official Symbol: UBD
• NCBI Official Synonym Symbols: FAT10; UBD-3; GABBR1
• NCBI Protein Information: ubiquitin D
• UniProt Protein Name: Ubiquitin D
• Protein Family: Ubiquitin
• UniProt Gene Name: UBD
• UniProt Synonym Gene Names: FAT10
• UniProt Entry Name: UBD_HUMAN

NCBI Description

This gene encodes a protein which contains two ubiquitin-like domains and appears to have similar function to ubiquitin. Through covalent attachment, the encoded protein targets other proteins for 26S proteasome degradation. This protein has been implicated to function in many cellular processes, including caspase-dependent apoptosis, formation of aggresomes, mitotic regulation, and dendritic cell maturation. Upregulation of this gene may promote inflammation in chronic kidney disease and has been observed in many cancer types. [provided by RefSeq, Aug 2017]

Uniprot Description

FAT10: Ubiquitin-like protein modifier which can be covalently attached to target protein and subsequently leads to their degradation by the 26S proteasome, in a NUB1L-dependent manner. Probably functions as a survival factor. Conjugation ability activated by UBA6. Promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). Regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. Required for TNF-alpha-induced p65 nuclear translocation in renal tubular epithelial cells (RTECs). May be involved in dendritic cell (DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T-cell responses. Mediates mitotic non-disjunction and chromosome instability, in long-term in vitro culture and cancers, by abbreviating mitotic phase and impairing the kinetochore localization of MAD2L1 during the prometaphase stage of the cell cycle. May be involved in the formation of aggresomes when proteasome is saturated or impaired. Mediates apoptosis in a caspase-dependent manner, especially in renal epithelium and tubular cells during renal diseases such as polycystic kidney disease and Human immunodeficiency virus (HIV)- associated nephropathy (HIVAN).

Protein type: Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: ubiquitin-dependent protein catabolic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein modification by small protein conjugation; positive regulation of apoptosis; protein ubiquitination; myeloid dendritic cell differentiation; proteolysis; response to organic nitrogen