Human Ubiquitin carboxyl-terminal hydrolase 2 (USP2) ELISA Kit | USP2 elisa kit

Human Ubiquitin carboxyl-terminal hydrolase 2 (USP2) ELISA Kit

Cat. Num. AAA287370

• Gene Names: USP2; USP9; UBP41
• Reactivity: Human
• Synonyms: Ubiquitin carboxyl-terminal hydrolase 2 (USP2); Human Ubiquitin carboxyl-terminal hydrolase 2 (USP2) ELISA Kit; UBP41; USP9; ubiquitin carboxyl-terminal hydrolase 2; ubiquitin specific protease 12; ubiquitin specific protease 2; ubiquitin specific protease 9; USP2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human USP2. No significant cross-reactivity or interference between Human USP2 and analogues was observed.
• Sequence Length: 362

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for USP2 elisa kit

This assay employs a two-site sandwich ELISA to quantitate USP2 in samples. An antibody specific for USP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any USP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for USP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of USP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 344313155
• NCBI GeneID: 9099
• NCBI Accession #: NP_001230688.1
• NCBI GenBank Nucleotide #: NM_001243759.1
• UniProt Accession #: O75604; Q8IUM2; Q8IW04; Q96MB9; Q9BQ21; B0YJB8; E9PPM2
• Molecular Weight: 45,241 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 2 isoform c
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 2
• NCBI Official Symbol: USP2
• NCBI Official Synonym Symbols: USP9; UBP41
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 2
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 2
• Protein Family: Protein
• UniProt Gene Name: USP2
• UniProt Synonym Gene Names: UBP41

NCBI Description

This gene encodes a member of the family of de-ubiquitinating enzymes, which belongs to the peptidase C19 superfamily. The encoded protein is a ubiquitin-specific protease which is required for TNF-alpha (tumor necrosis factor alpha) -induced NF-kB (nuclear factor kB) signaling. This protein deubiquitinates polyubiquitinated target proteins such as fatty acid synthase, murine double minute 2 (MDM2), MDM4/MDMX and cyclin D1. MDM2 and MDM4 are negative regulators of the p53 tumor suppressor and cyclin D1 is required for cell cycle G1/S transition. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1 (PubMed:17290220, PubMed:19917254, PubMed:19838211). Isoform 1 and isoform 4 possess both ubiquitin-specific peptidase and isopeptidase activities (). Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity (PubMed:17290220, PubMed:19838211). Has no deubiquitinase activity against p53/TP53 (PubMed:17290220). Prevents MDM2-mediated degradation of MDM4 (PubMed:17290220). Plays a role in the G1/S cell-cycle progression in normal and cancer cells (PubMed:19917254). Regulates the circadian clock by modulating its intrinsic circadian rhythm and its capacity to respond to external cues (). Associates with clock proteins and deubiquitinates core clock component PER1 but does not affect its overall stability (). Regulates the nucleocytoplasmic shuttling and nuclear retention of PER1 and its repressive role on the clock transcription factors CLOCK and ARNTL/BMAL1 (). Plays a role in the regulation of myogenic differentiation of embryonic muscle cells ().