Human Ub ELISA Kit | Ub elisa kit

Human Ub (Ubiquitin) ELISA Kit

Cat. Num. AAA2505642

• Gene Names: Ubi-p63E; CG11624; DmelCG11624; DmUb; DmUbi-p63E; magn; Ub; UB; ubi; Ubi-p; ubi-p63E; Ubi-P63e; ubi63E; Ubi63E; Ubi63p; Ubip; Ubip63E; Ubiq; Ubp
• Reactivity: Human
• Synonyms: Ub; Human Ub (Ubiquitin) ELISA Kit; Ub elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human Ub in samples. No significant cross-reactivity or interference between Human Ub and analogues was observed.
• Sequence Length: 763

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5-4000pg/mL
• Sensitivity: 37.5pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human Ub were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human Ub were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Ub elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human Ub concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Ub. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Ub and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Ub, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human Ub. You can calculate the concentration of Human Ub in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 442630045
• NCBI GeneID: 38456
• NCBI Accession #: NP_001261383.1
• NCBI GenBank Nucleotide #: NM_001274454.1
• UniProt Accession #: P0CG69; P68198; Q0E8I1; Q9VKW6; Q9VQX7; Q9VZL4
• Molecular Weight: 85,799 Da
• NCBI Official Full Name: Ubiquitin-63E, isoform D
• NCBI Official Synonym Full Names: Ubiquitin-63E
• NCBI Official Symbol: Ubi-p63E
• NCBI Official Synonym Symbols: CG11624; DmelCG11624; DmUb; DmUbi-p63E; magn; Ub; UB; ubi; Ubi-p; ubi-p63E; Ubi-P63e; ubi63E; Ubi63E; Ubi63p; Ubip; Ubip63E; Ubiq; Ubp
• NCBI Protein Information: CG11624 gene product from transcript CG11624-RB; CG11624-PA; CG11624-PB; CG11624-PC; CG11624-PD; Ubi-p63E-PA; Ubi-p63E-PB; Ubi-p63E-PC; Ubi-p63E-PD; Ubiquitin-63-E; Ubiquitin-63E; Ubiquitin-polymeric; magellan; polyubiquitin; ubiquitin 63E
• UniProt Protein Name: Polyubiquitin
• Protein Family: Ubiquilin
• UniProt Gene Name: Ubi-p63E
• UniProt Synonym Gene Names: Ubi-m
• UniProt Entry Name: UBIQP_DROME

Uniprot Description

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-48-linked is involved in protein degradation via the proteasome. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling ().