Human U2AF1 (Splicing factor U2AF 35 kDa subunit) ELISA Kit | U2AF1 elisa kit

Human U2AF1 (Splicing factor U2AF 35 kDa subunit) ELISA Kit

Cat. Num. AAA7612207

• Gene Names: U2AF1; RN; FP793; U2AF35; U2AFBP; RNU2AF1
• Reactivity: Human
• Synonyms: U2AF1 (Splicing factor U2AF 35 kDa subunit); Human U2AF1 (Splicing factor U2AF 35 kDa subunit) ELISA Kit; Splicing factor U2AF 35 kDa subunit; U2 auxiliary factor 35 kDa subunit; U2 small nuclear RNA auxiliary factor 1; U2 snRNP auxiliary factor small subunit; U2AF35; U2AFBP; FP793; U2AF1; U2AF1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of U2AF1. No significant cross-reactivity or interference between U2AF1 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for U2AF1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 267187
• NCBI GeneID: 7307
• UniProt Accession #: Q01081; Q701P4; Q71RF1
• Molecular Weight: 240
• NCBI Official Full Name: Splicing factor U2AF 35 kDa subunit
• NCBI Official Synonym Full Names: U2 small nuclear RNA auxiliary factor 1
• NCBI Official Symbol: U2AF1
• NCBI Official Synonym Symbols: RN; FP793; U2AF35; U2AFBP; RNU2AF1
• NCBI Protein Information: splicing factor U2AF 35 kDa subunit; splicing factor U2AF 35kDa subunit; U2 small nuclear RNA auxillary factor 1; U2 snRNP auxiliary factor small subunit; U2(RNU2) small nuclear RNA auxiliary factor binding protein; U2 small nuclear ribonucleoprotein auxi
• UniProt Protein Name: Splicing factor U2AF 35 kDa subunit
• Protein Family: Splicing factor
• UniProt Gene Name: U2AF1
• UniProt Synonym Gene Names: U2AF35; U2AFBP
• UniProt Entry Name: U2AF1_HUMAN

NCBI Description

This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

U2AF1: Plays a critical role in both constitutive and enhancer- dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron. Belongs to the splicing factor SR family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Spliceosome; RNA splicing; RNA-binding; RNA processing

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: nucleoplasm; spliceosome; Cajal body; nuclear speck

Molecular Function: RS domain binding; protein binding; metal ion binding; nucleotide binding

Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; RNA splicing; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription