Typical Testing Data/Standard Curve (for reference only)
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Human Tyrosine protein kinase Yes (YES1) ELISA Kit | YES1 elisa kit
Human Tyrosine protein kinase Yes (YES1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
()
Typical Testing Data/Standard Curve (for reference only)
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Principle of the Assay: YES1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-YES1 antibody and an YES1-HRP conjugate. The assay sample and buffer are incubated together with YES1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the YES1 concentration since YES1 from samples and YES1-HRP conjugate compete for the anti-YES1 antibody binding site. Since the number of sites is limited, as more sites are occupied by YES1 from the sample, fewer sites are left to bind YES1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The YES1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene is the cellular homolog of the Yamaguchi sarcoma virus oncogene. The encoded protein has tyrosine kinase activity and belongs to the src family of proteins. This gene lies in close proximity to thymidylate synthase gene on chromosome 18, and a corresponding pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008]
Uniprot Description
Yes: a proto-oncogenic cytosolic tyrosine kinase of the Src family. Expressed at high levels in adult neurons, spermatozoa, platelets, and epithelial cells.
Protein type: EC 2.7.10.2; Kinase, protein; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group; Src family
Chromosomal Location of Human Ortholog: 18p11.31-p11.21
Cellular Component: Golgi apparatus; extrinsic to internal side of plasma membrane; focal adhesion; cytoplasm; plasma membrane; microtubule organizing center; actin filament; cytosol
Molecular Function: protein binding; enzyme binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; epidermal growth factor receptor binding; receptor binding
Biological Process: axon guidance; T cell costimulation; ephrin receptor signaling pathway; innate immune response; protein modification process; regulation of vascular permeability; glucose transport; cell differentiation; blood coagulation; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation
Research Articles on YES1
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Product Notes
The Human YES1 yes1 (Catalog #AAA7218835) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7218835 ELISA Kit recognizes Human YES1. It is sometimes possible for the material contained within the vial of "Tyrosine protein kinase Yes (YES1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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