Mouse anti-Human CALR / Calreticulin Monoclonal Antibody | anti-CALR antibody

Mouse Monoclonal [clone 1G11-1A9] (IgG1,k) to Human CALR / Calreticulin

Cat. Num. AAA246463

• Gene Names: CALR; RO; CRT; SSA; cC1qR; HEL-S-99n
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: CALR / Calreticulin; Monoclonal Antibody; Mouse Monoclonal [clone 1G11-1A9] (IgG1; k) to Human CALR / Calreticulin; Anti-CALR / Calreticulin Antibody (clone 1G11-1A9) IHC-plus; CALR; Calregulin; CC1qR; CRP55; CRT; CRTC; ERp60; Grp60; RO; SSA; Calreticulin; HACBP; Human CALR; anti-CALR antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1G11-1A9
• Specificity: Human Calreticulin
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Concentration: 0.22 mg/ml (varies by lot)
• Sequence Length: 417

• Applicable Applications for anti-CALR antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB; Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
• Target Species: Human
• Immunogen Description: CALR (AAH02500, 1 a.a. ~ 339 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: CALR / Calreticulin antibody was raised against cALR (AAH02500, 1 a.a. ~ 339 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-Calreticulin antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of CALR expression in transfected 293T cell line by CALR monoclonal antibody (M01), clone 1G11-1A9.)

Western Blot (WB)

(Western blot of CALR expression in K-562 by CALR monoclonal antibody (M01), clone 1G11-1A9.)

Related Product Information for anti-CALR antibody

Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors.

NCBI and Uniprot Product Information

• NCBI GI #: 4757900
• NCBI GeneID: 811
• NCBI Accession #: NP_004334.1
• NCBI GenBank Nucleotide #: NM_004343.3
• UniProt Accession #: P27797; Q6IAT4; Q9UDG2
• Molecular Weight: 48,142 Da
• NCBI Official Full Name: calreticulin
• NCBI Official Synonym Full Names: calreticulin
• NCBI Official Symbol: CALR
• NCBI Official Synonym Symbols: RO; CRT; SSA; cC1qR; HEL-S-99n
• NCBI Protein Information: calreticulin; CRP55; ERp60; HACBP; grp60; calregulin; endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 99n; Sicca syndrome antigen A (autoantigen Ro; calreticulin)
• UniProt Protein Name: Calreticulin
• UniProt Gene Name: CALR
• UniProt Synonym Gene Names: CRTC; ERp60
• UniProt Entry Name: CALR_HUMAN

NCBI Description

Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes. [provided by RefSeq, Jul 2008]

Uniprot Description

Calreticulin: Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis. Monomer. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with PDIA3/ERp57. Interacts with NR3C1 and TRIM21. Interacts with GABARAP. Belongs to the calreticulin family.

Protein type: Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Secreted; Calcium-binding; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: Golgi apparatus; extracellular space; cell surface; focal adhesion; sarcoplasmic reticulum lumen; smooth endoplasmic reticulum; endoplasmic reticulum lumen; endoplasmic reticulum; extracellular region; acrosome; cytosol; polysome; proteinaceous extracellular matrix; membrane; perinuclear region of cytoplasm; cytoplasm; intracellular; nucleus; external side of plasma membrane

Molecular Function: hormone binding; zinc ion binding; complement component C1q binding; unfolded protein binding; calcium ion binding; peptide binding; mRNA binding; integrin binding; protein binding; DNA binding; androgen receptor binding; iron ion binding; chaperone binding; ubiquitin protein ligase binding; glycoprotein binding; carbohydrate binding

Biological Process: protein maturation via protein folding; protein folding; regulation of meiosis; negative regulation of transcription from RNA polymerase II promoter; cardiac muscle cell differentiation; response to estradiol stimulus; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of transcription, DNA-dependent; positive regulation of phagocytosis; positive regulation of cell proliferation; antigen processing and presentation of exogenous peptide antigen via MHC class I; protein export from nucleus; cortical actin cytoskeleton organization and biogenesis; cell cycle arrest; response to drug; receptor-mediated endocytosis; negative regulation of steroid hormone receptor signaling pathway; protein stabilization; unfolded protein response; positive regulation of cell cycle; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; response to testosterone stimulus; post-translational protein modification; peptide antigen assembly with MHC class I protein complex; cellular calcium ion homeostasis; negative regulation of retinoic acid receptor signaling pathway; negative regulation of neuron differentiation; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; negative regulation of translation; sequestering of calcium ion; glucocorticoid receptor signaling pathway; spermatogenesis; protein amino acid N-linked glycosylation via asparagine; negative regulation of transcription, DNA-dependent; positive regulation of DNA replication

Disease: Myelofibrosis; Thrombocythemia 1