Human Tubulin Beta 1 (TUBb1) ELISA Kit | TUBb1 elisa kit

Human Tubulin Beta 1 (TUBb1) ELISA Kit

Cat. Num. AAA457225

• Reactivity: Human
• Synonyms: Tubulin Beta 1 (TUBb1); Human Tubulin Beta 1 (TUBb1) ELISA Kit; TUBb1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TUBb1. No significant cross-reactivity or interference between TUBb1 and analogues was observed.
• Sequence Length: 451

• Samples: Tissue homogenates, cell lysates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/mL
• Sensitivity: <30.8pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TUBb1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TUBb1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TUBb1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of TUBb1 in human tissue homogenates, cell lysates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TUBb1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TUBb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TUBb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TUBb1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13562114
• NCBI GeneID: 81027
• NCBI Accession #: NP_110400.1
• NCBI GenBank Nucleotide #: NM_030773.3
• UniProt Accession #: Q9H4B7
• Molecular Weight: 50,327 Da
• NCBI Official Full Name: tubulin beta-1 chain
• NCBI Official Synonym Full Names: tubulin beta 1 class VI
• NCBI Official Symbol: TUBB1
• NCBI Protein Information: tubulin beta-1 chain
• UniProt Protein Name: Tubulin beta-1 chain
• Protein Family: Tubulin
• UniProt Gene Name: TUBB1

NCBI Description

This gene encodes a member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in this gene is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of this gene are found on chromosome Y.[provided by RefSeq, Jul 2010]

Uniprot Description

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain ().