Human TSC2 (Tuberous Sclerosis Protein 2) ELISA Kit | TSC2 elisa kit

Human TSC2 (Tuberous Sclerosis Protein 2) ELISA Kit

Cat. Num. AAA7606852

• Gene Names: TSC2; LAM; TSC4; PPP1R160
• Reactivity: Human
• Synonyms: TSC2 (Tuberous Sclerosis Protein 2); Human TSC2 (Tuberous Sclerosis Protein 2) ELISA Kit; TSC2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TSC2. No significant cross-reactivity or interference between TSC2 and analogues was observed.
• Sequence Length: 1807

• Samples: Cell, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Intra-assay Precision: Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TSC2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 269849475
• NCBI GeneID: 7249
• UniProt Accession #: P49815; O75275; Q4LE71; Q8TAZ1; A7E2E2; B4DIL8; B4DIQ7; B4DRN2; B7Z2B8; C9J378
• Molecular Weight: 25,773 Da
• NCBI Official Full Name: Tuberin
• NCBI Official Synonym Full Names: tuberous sclerosis 2
• NCBI Official Symbol: TSC2
• NCBI Official Synonym Symbols: LAM; TSC4; PPP1R160
• NCBI Protein Information: tuberin
• UniProt Protein Name: Tuberin
• Protein Family: Tuberin
• UniProt Gene Name: TSC2
• UniProt Synonym Gene Names: TSC4

NCBI Description

Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

TSC2: a product of the tumor suppressor gene TSC2. Tuberin and Hamartin (TSC1) form a tumor suppressor heterodimer that inhibits the mTOR nutrient signaling input. TSC1/TSC2 targets the small G protein Rheb, a novel mediator of the nutrient signaling input to mTOR. Functions as a Rheb GTPase activating protein (GAP). Four alternatively spliced isoforms have been described.

Protein type: Cell cycle regulation; GAP; GAP, misc.; Nuclear receptor co-regulator; Tumor suppressor

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: cytoplasm; cytosol; Golgi apparatus; lysosome; membrane; nucleus; perinuclear region of cytoplasm; TSC1-TSC2 complex

Molecular Function: GTPase activator activity; phosphatase binding; protein binding; protein homodimerization activity; small GTPase binding

Biological Process: anoikis; cell cycle arrest; endocytosis; heart development; insulin-like growth factor receptor signaling pathway; negative regulation of cell proliferation; negative regulation of insulin receptor signaling pathway; negative regulation of phosphoinositide 3-kinase cascade; negative regulation of protein kinase activity; negative regulation of protein kinase B signaling cascade; negative regulation of TOR signaling pathway; negative regulation of Wnt receptor signaling pathway; neural tube closure; positive chemotaxis; positive regulation of macroautophagy; protein import into nucleus; protein kinase B signaling cascade; protein localization; regulation of cell cycle; regulation of endocytosis; regulation of insulin receptor signaling pathway; vesicle-mediated transport

Disease: Focal Cortical Dysplasia Of Taylor; Lymphangioleiomyomatosis; Tuberous Sclerosis 2