Typical Testing Data/Standard Curve (for reference only)
Human Tropomyosin Alpha 1 chain (TPM1) ELISA Kit | TPM1 elisa kit
Human Tropomyosin Alpha 1 chain (TPM1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: TPM1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-TPM1 antibody and an TPM1-HRP conjugate. The assay sample and buffer are incubated together with TPM1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TPM1 concentration since TPM1 from samples and TPM1-HRP conjugate compete for the anti-TPM1 antibody binding site. Since the number of sites is limited, as more sites are occupied by TPM1 from the sample, fewer sites are left to bind TPM1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TPM1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]
Uniprot Description
TPM1: a cytoskeletal protein that binds to actin filaments in muscle and nonmuscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Defects in TPM3 are a cause of nemaline myopathy type 1 (NEM1). Three alternatively spliced isoforms have been described.
Protein type: Actin-binding; Motility/polarity/chemotaxis; Motor
Chromosomal Location of Human Ortholog: 15q22.1
Cellular Component: filamentous actin; sarcomere; cytoskeleton; stress fiber; muscle thin filament tropomyosin; cytosol
Molecular Function: structural constituent of cytoskeleton; structural constituent of muscle; cytoskeletal protein binding; actin binding
Biological Process: positive regulation of cell adhesion; wound healing; regulation of muscle contraction; in utero embryonic development; cytoskeleton organization and biogenesis; sarcomere organization; regulation of heart contraction; muscle filament sliding; muscle contraction; positive regulation of ATPase activity; positive regulation of stress fiber formation; ruffle organization and biogenesis; ventricular cardiac muscle morphogenesis; cell motility; negative regulation of cell migration; positive regulation of heart rate by epinephrine; cardiac muscle contraction
Disease: Cardiomyopathy, Dilated, 1y; Cardiomyopathy, Familial Hypertrophic, 3
Research Articles on TPM1
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Product Notes
The Human TPM1 tpm1 (Catalog #AAA7250291) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7250291 ELISA Kit recognizes Human TPM1. It is sometimes possible for the material contained within the vial of "Tropomyosin Alpha 1 chain (TPM1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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