Typical Testing Data/Standard Curve (for reference only)
Human Tripartite Motif-Containing Protein 35, TRIM35 ELISA Kit | TRIM35 elisa kit
Human Tripartite Motif-Containing Protein 35, TRIM35 ELISA Kit
Gene Names
TRIM35; HLS5; MAIR
Reactivity
Human
Synonyms
Tripartite Motif-Containing Protein 35; TRIM35; Human Tripartite Motif-Containing Protein 35; TRIM35 ELISA Kit; TRIM35 elisa kit
Reactivity
Human
Sequence Length
493
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
10ng/L - 2000ng/L
Sensitivity
4.93ng/L
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TRIM35 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Tripartite Motif-containing Protein 35 (also known as TRIM35) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human TRIM35 antibody. TRIM35 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TRIM35 Antibody is added and binds to TRIM35 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TRIM35 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human TRIM35. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human TRIM35 antibody. TRIM35 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TRIM35 Antibody is added and binds to TRIM35 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TRIM35 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human TRIM35. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tripartite motif-containing protein 35 isoform 1
NCBI Official Synonym Full Names
tripartite motif containing 35
NCBI Official Symbol
TRIM35
NCBI Official Synonym Symbols
HLS5; MAIR
NCBI Protein Information
tripartite motif-containing protein 35
UniProt Protein Name
Tripartite motif-containing protein 35
Protein Family
UniProt Gene Name
TRIM35
UniProt Synonym Gene Names
HLS5; KIAA1098
NCBI Description
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The function of this protein has not been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
TRIM35: May play a role as a tumor suppressor. Implicated in the cell death mechanism. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 8p21.2
Cellular Component: cytoplasm; nucleus
Biological Process: apoptosis; innate immune response; negative regulation of mitotic cell cycle; positive regulation of apoptosis
Research Articles on TRIM35
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Product Notes
The Human TRIM35 trim35 (Catalog #AAA1604129) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1604129 ELISA Kit recognizes Human TRIM35. It is sometimes possible for the material contained within the vial of "Tripartite Motif-Containing Protein 35, TRIM35, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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