Human Transmembrane Protein 106B (TMEM106B) ELISA Kit | TMEM106B elisa kit

Human Transmembrane Protein 106B (TMEM106B) ELISA Kit

Cat. Num. AAA2707797

• Reactivity: Human
• Synonyms: Transmembrane Protein 106B (TMEM106B); Human Transmembrane Protein 106B (TMEM106B) ELISA Kit; TMEM106B elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TMEM106B. No significant cross-reactivity or interference between TMEM106B and analogues was observed.
• Sequence Length: 274

• Samples: Human tissue homogenates, cell lysates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.115ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TMEM106B were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TMEM106B were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TMEM106B elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TMEM106B in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TMEM106B. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TMEM106B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TMEM106B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TMEM106B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 109895058
• UniProt Accession #: Q9NUM4
• NCBI Official Full Name: Transmembrane protein 106B
• UniProt Protein Name: Transmembrane protein 106B
• Protein Family: Transmembrane protein
• UniProt Gene Name: TMEM106B
• UniProt Entry Name: T106B_HUMAN

Uniprot Description

TMEM106B: TMEM106B genotype, when containing 3 particular single-nucleotide polymorphisms, is strongly correlated with frontotemporal lobar degeneration with TAR DNA-binding protein (TDP-43) inclusions (FTLD-TDP). Frontotemporal lobar degeneration (FTLD) is the second most common cause of presenile dementia and 20% of patients with this neurodegenerative disease have autosomal dominant GRN mutations. Expression of TMEM106B associated with these polymorphisms is increased in frontal cortex of patients with FTLD-TDP compared to unaffected controls. Thus, increased TMEM106B expression in the brain may be linked to mechanisms of disease in FTLD-TDP and risk alleles confer genetic susceptibility by increasing gene expression. Belongs to the TMEM106 family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 7p21.3

Cellular Component: intracellular membrane-bound organelle; lysosomal membrane; late endosome membrane; integral to membrane

Molecular Function: protein binding

Biological Process: lysosome localization; transport; dendrite morphogenesis