Goat PSMB10 Polyclonal Antibody | anti-PSMB10 antibody

Goat Polyclonal to Human PSMB10

Cat. Num. AAA248321

• Gene Names: PSMB10; LMP10; MECL1; beta2i
• Reactivity: Gibbon, Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: PSMB10; Polyclonal Antibody; Goat Polyclonal to Human PSMB10; Anti-PSMB10 Antibody (Internal) IHC-plus; Beta2i; Low molecular mass protein 10; MECL1; Proteasome component mecl-1; Proteasome subunit beta-2i; Proteasome subunit beta 7i; Macropain subunit MECl-1; Proteasome MECl-1; LMP10; Proteasome subunit MECL1; Human PSMB10; anti-PSMB10 antibody

• Host: Goat
• Reactivity: Gibbon, Human
• Clonality: Polyclonal
• Specificity: Human PSMB10.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Sequence Length: 273

• Applicable Applications for anti-PSMB10 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:8000), IHC-P (5 ug/ml), WB (0.3 - 1 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 26kDa band observed in Human, Liver, Lung and Lymph Node lysates (calculated MW of 28.9kDa according to NP_002792.1). Recommended concentration: 0.3-1 ug/ml.
• Target Species: Human
• Immunogen Description: Synthetic peptide C-PTEPVKRSGRYH from an internal region of human PSMB10 (NP_002792.1). Percent identity by BLAST analysis: Human, Gibbon (100%); Gorilla, Monkey, Marmoset (92%); Elephant (83%).
• Immunogen Type: Synthetic peptide
• Immunogen: PSMB10 antibody was raised against synthetic peptide C-PTEPVKRSGRYH from an internal region of human PSMB10 (NP_002792.1). Percent identity by BLAST analysis: Human, Gibbon (100%); Gorilla, Monkey, Marmoset (92%); Elephant (83%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-PSMB10 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-PSMB10 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(PSMB10 antibody (0.3 ug/ml) staining of Human Lung lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Product Categories/Family for anti-PSMB10 antibody

Family: Protease Non Catalytic; Subfamily: Threonine T1

NCBI and Uniprot Product Information

• NCBI GI #: 4506191
• NCBI GeneID: 5699
• NCBI Accession #: NP_002792.1
• NCBI GenBank Nucleotide #: NM_002801.3
• UniProt Accession #: P40306; Q5U098; B2R5J4
• Molecular Weight: 28,936 Da
• NCBI Official Full Name: proteasome subunit beta type-10
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) subunit, beta type, 10
• NCBI Official Symbol: PSMB10
• NCBI Official Synonym Symbols: LMP10; MECL1; beta2i
• NCBI Protein Information: proteasome subunit beta type-10; proteasome MECl-1; macropain subunit MECl-1; proteasome subunit MECL1; proteasome subunit beta 7i; proteasome subunit beta-2i; low molecular mass protein 10; proteasome catalytic subunit 2i; multicatalytic endopeptidase complex subunit MECl-1
• UniProt Protein Name: Proteasome subunit beta type-10
• Protein Family: Proteasome
• UniProt Gene Name: PSMB10
• UniProt Synonym Gene Names: LMP10; MECL1
• UniProt Entry Name: PSB10_HUMAN

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome. [provided by RefSeq, Jul 2008]

Uniprot Description

PSMB10: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Belongs to the peptidase T1B family.

Protein type: Proteasome complex; EC 3.4.25.1; Protease

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: nucleoplasm; proteasome complex; proteasome core complex; cytosol

Molecular Function: threonine endopeptidase activity

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; cell morphogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; humoral immune response; regulation of apoptosis; T cell proliferation; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis