Human Transglutaminase 1, Keratinocyte (TGM1) ELISA Kit | TGM1 elisa kit

Human Transglutaminase 1, Keratinocyte (TGM1) ELISA Kit

Cat. Num. AAA455003

• Gene Names: TGM1; LI; KTG; LI1; TGK; ICR2; ARCI1; TGASE
• Reactivity: Human
• Synonyms: Transglutaminase 1; Keratinocyte (TGM1); Human Transglutaminase 1; Keratinocyte (TGM1) ELISA Kit; ISGF-3; P113; STAT113; TGM1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TGM1. No significant cross-reactivity or interference between TGM1 and analogues was observed.
• Sequence Length: 817

• Samples: Human tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: <0.121ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TGM1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TGM1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TGM1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of TGM1 in human tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGM1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TGM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TGM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TGM1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4507475
• NCBI GeneID: 7051
• NCBI Accession #: NP_000350.1
• NCBI GenBank Nucleotide #: NM_000359.2
• UniProt Accession #: P22735; Q197M4; B4DWR7
• Molecular Weight: 40,628 Da
• NCBI Official Full Name: protein-glutamine gamma-glutamyltransferase K
• NCBI Official Synonym Full Names: transglutaminase 1
• NCBI Official Symbol: TGM1
• NCBI Official Synonym Symbols: LI; KTG; LI1; TGK; ICR2; ARCI1; TGASE
• NCBI Protein Information: protein-glutamine gamma-glutamyltransferase K
• UniProt Protein Name: Protein-glutamine gamma-glutamyltransferase K
• Protein Family: Protein-glutamine gamma-glutamyltransferase
• UniProt Gene Name: TGM1
• UniProt Synonym Gene Names: KTG; TG(K); TGK; TGase K; TGase-1

NCBI Description

The protein encoded by this gene is a membrane protein that catalyzes the addition of an alkyl group from an akylamine to a glutamine residue of a protein, forming an alkylglutamine in the protein. This protein alkylation leads to crosslinking of proteins and catenation of polyamines to proteins. This gene contains either one or two copies of a 22 nt repeat unit in its 3' UTR. Mutations in this gene have been associated with autosomal recessive lamellar ichthyosis (LI) and nonbullous congenital ichthyosiform erythroderma (NCIE). [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins. Responsible for cross-linking epidermal proteins during formation of the stratum corneum. Involved in cell proliferation (PubMed:26220141).