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Typical Testing Data/Standard Curve (for reference only) ()

Human Transcription factor 7 (TCF7) ELISA Kit | TCF7 elisa kit

Human Transcription factor 7 (TCF7) ELISA Kit

Gene Names
Tcf7; Tcf1; TCF-1; AI465550
Reactivity
Human
Synonyms
Transcription factor 7 (TCF7); Human Transcription factor 7 (TCF7) ELISA Kit; TCF7 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Sequence Length
303
Samples
Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.1-40ng/ml
Sensitivity
0.054ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

()

Typical Testing Data/Standard Curve (for reference only) ()
Related Product Information for TCF7 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Transcription factor 7 (also known as TCF7) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human TCF7 antibody. TCF7 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TCF7 Antibody is added and binds to TCF7 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TCF7 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human TCF7. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,609 Da
NCBI Official Full Name
transcription factor 7
NCBI Official Synonym Full Names
transcription factor 7, T cell specific
NCBI Official Symbol
Tcf7
NCBI Official Synonym Symbols
Tcf1; TCF-1; AI465550
NCBI Protein Information
transcription factor 7
UniProt Protein Name
Transcription factor 7
Protein Family
UniProt Gene Name
Tcf7
UniProt Synonym Gene Names
Tcf-1; Tcf1; TCF-7; T-cell factor 1; TCF-1

NCBI Description

This gene encodes a transcription factor which is a member of the T-cell specific transcription factor family. The encoded protein is distinct from the hepatic transcription factor, transcription factor 1, which is also referred to by the symbol Tcf1. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Sep 2015]

Uniprot Description

Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4+ CD8+ immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1 ().

Research Articles on TCF7

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Product Notes

The Human TCF7 tcf7 (Catalog #AAA166776) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA166776 ELISA Kit recognizes Human TCF7. It is sometimes possible for the material contained within the vial of "Transcription factor 7 (TCF7), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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