Human TPH1 ELISA Kit | TPH1 elisa kit

Human TPH1(Tryptophan Hydroxylase 1)ELISA Kit

Cat. Num. AAA2510541

• Gene Names: TPH1; TPRH; TRPH
• Reactivity: Human
• Synonyms: TPH1; Human TPH1(Tryptophan Hydroxylase 1)ELISA Kit; TPH1 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant Human TPH1. No significant cross-reactivity or interference between Human TPH1 and analogues was observed.
• Sequence Length: 444

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human TPH1 concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TPH1 elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TPH1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TPH1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TPH1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TPH1. You can calculate the concentration of TPH1 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 76826965
• NCBI GeneID: 7166
• UniProt Accession #: P17752; O95188; O95189; Q16736; Q3KPG8; D3DQX6
• Molecular Weight: 53,394 Da
• NCBI Official Full Name: Tryptophan hydroxylase 1
• NCBI Official Synonym Full Names: tryptophan hydroxylase 1
• NCBI Official Symbol: TPH1
• NCBI Official Synonym Symbols: TPRH; TRPH
• NCBI Protein Information: tryptophan 5-hydroxylase 1; L-tryptophan hydroxylase; indoleacetic acid-5-hydroxylase; tryptophan 5-monooxygenase 1; tryptophan hydroxylase (tryptophan 5-monooxygenase)
• UniProt Protein Name: Tryptophan 5-hydroxylase 1
• Protein Family: Tryptophan 5-hydroxylase
• UniProt Gene Name: TPH1
• UniProt Synonym Gene Names: TPH; TPRH; TRPH
• UniProt Entry Name: TPH1_HUMAN

NCBI Description

This gene encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others.[provided by RefSeq, Apr 2009]

Uniprot Description

TPH1: an enzyme that is rate-limiting in the biosynthesis of serotonin. It catalyzes the biopterin dependent monooxygenation of tryptophan to 5-hydroxytryptophan (5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. Its expression is limited to a few specialized tissues: raphe neurons, pinealocytes, mast cells, mononuclear leukocytes, beta-cells of the islets of Langerhans, and intestinal and pancreatic enterochromaffin cells. Two alternatively spliced isoforms have been described.

Protein type: EC 1.14.16.4; Amino Acid Metabolism - tryptophan; Oxidoreductase

Chromosomal Location of Human Ortholog: 11p15.3-p14

Cellular Component: neuron projection; cytosol

Molecular Function: amino acid binding; tryptophan 5-monooxygenase activity; iron ion binding

Biological Process: circadian rhythm; aromatic amino acid family metabolic process; indolalkylamine biosynthetic process; negative regulation of ossification; positive regulation of fat cell differentiation; bone remodeling; serotonin biosynthetic process