Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human TOM1-like protein 2 (TOM1L2) Standard Curve.)
Human TOM1-Like Protein 2 (TOM1L2) ELISA Kit | TOM1L2 elisa kit
Human TOM1-Like Protein 2 (TOM1L2) ELISA Kit
Reactivity
Human
Synonyms
TOM1-Like Protein 2 (TOM1L2); Human TOM1-Like Protein 2 (TOM1L2) ELISA Kit; TOM1L2; FLJ32746; target of myb1-like 2; TOM1L2 elisa kit
Reactivity
Human
Specificity
Human TOM1-like protein 2 (TOM1L2) ELISA Kit has high sensitivity and excellent specificity for detection of Human TOM1L2. No significant cross-reactivity or interference between Human TOM1L2 and analogues was observed.
Sequence Length
507
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human TOM1-like protein 2 (TOM1L2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human TOM1-like protein 2 (TOM1L2) Standard Curve.)
Related Product Information for TOM1L2 elisa kit
Description: This Human TOM1-like protein 2 (TOM1L2) ELISA Kit employs a two-site sandwich ELISA to quantitate TOM1L2 in samples. An antibody specific for TOM1L2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TOM1L2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOM1L2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOM1L2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The N-terminal domain of TOM1 shares sequence similarity with the N-terminal domains of human HGS, human STAM, and yeast VPS27, all of which are proteins associated with vesicular trafficking at the endosome. By searching sequence databases using TOM1 as the query, Seroussi et al. (1999) identified TOM1L1 as another protein with an N-terminal domain similar to that of TOM1. The authors assembled the sequences of a group of human TOM1L1 ESTs into a contiguous cDNA encoding a deduced 476-amino acid protein. TOM1L1 shares 37% sequence identity with TOM1; its C-terminal domain is not similar to that of TOM1.
Background: The N-terminal domain of TOM1 shares sequence similarity with the N-terminal domains of human HGS, human STAM, and yeast VPS27, all of which are proteins associated with vesicular trafficking at the endosome. By searching sequence databases using TOM1 as the query, Seroussi et al. (1999) identified TOM1L1 as another protein with an N-terminal domain similar to that of TOM1. The authors assembled the sequences of a group of human TOM1L1 ESTs into a contiguous cDNA encoding a deduced 476-amino acid protein. TOM1L1 shares 37% sequence identity with TOM1; its C-terminal domain is not similar to that of TOM1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
TOM1-like protein 2 isoform 3
NCBI Official Synonym Full Names
target of myb1 like 2 membrane trafficking protein
NCBI Official Symbol
TOM1L2
NCBI Protein Information
TOM1-like protein 2
UniProt Protein Name
TOM1-like protein 2
Protein Family
UniProt Gene Name
TOM1L2
NCBI Description
This gene belongs to a small gene family whose members have an N-terminal VHS domain followed by a GAT domain; domains which typically participate in vesicular trafficking. The canonical protein encoded by this gene also has a C-terminal clathrin binding motif. This protein has been shown to interact with Tollip, clathrin and ubiquitin and is thought to play a role in endosomal sorting. This gene resides in the 3.7 Mb deletion of chromosome region 17p11.2 that is associated with Smith-Magenis syndrome. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Apr 2017]
Uniprot Description
Probable role in protein transport. May regulate growth factor-induced mitogenic signaling.
Research Articles on TOM1L2
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Product Notes
The Human TOM1L2 tom1l2 (Catalog #AAA9713835) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713835 ELISA Kit recognizes Human TOM1L2. It is sometimes possible for the material contained within the vial of "TOM1-Like Protein 2 (TOM1L2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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