Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Thymosin beta-4 (TMSB4X) Standard Curve.)
Human Thymosin beta-4 (TMSB4X) ELISA Kit | TMSB4X elisa kit
Human Thymosin beta-4 (TMSB4X) ELISA Kit
Gene Names
TMSB4X; FX; TB4X; PTMB4; TMSB4
Reactivity
Human
Synonyms
Thymosin beta-4 (TMSB4X); Human Thymosin beta-4 (TMSB4X) ELISA Kit; TMSB4X; FX; PTMB4; TB4X; TMSB4; OTTHUMP00000022925; OTTHUMP00000022927; OTTHUMP00000022928; prothymosin beta-4; thymosin beta-4; thymosin; beta 4; X chromosome; TMSB4X elisa kit
Reactivity
Human
Specificity
Human Thymosin beta-4 (TMSB4X) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMSB4X. No significant cross-reactivity or interference between Human TMSB4X and analogues was observed.
Sequence Length
44
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Thymosin beta-4 (TMSB4X) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Thymosin beta-4 (TMSB4X) Standard Curve.)
Related Product Information for TMSB4X elisa kit
Description: This Human Thymosin beta-4 (TMSB4X) ELISA Kit employs a two-site sandwich ELISA to quantitate TMSB4X in samples. An antibody specific for TMSB4X has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMSB4X present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMSB4X is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMSB4X bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The predominant form of thymosin, thymosin beta4, is a member of a highly conserved family of actin monomer-sequestering proteins. Beta-thymosins are the primary regulators of unpolymerized actin, and are essential for maintaining the small cytoplasmic pool of free G-actin monomers required for rapid filament elongation and allowing for the flux of monomers between the thymosin-bound pool and F-actin. Thymosin beta4 sequesters actin, holding it in a form that is unable to polymerize. Due to its profusion in the cytosol and its ability to bind ATP G-actin but not F-actin, thymosin beta4 is regarded as the principal actin-sequestering protein. Thymosin beta4 binds ATP G- monomeric actin in a 11 complex where G-actin cannot polymerize.
Background: The predominant form of thymosin, thymosin beta4, is a member of a highly conserved family of actin monomer-sequestering proteins. Beta-thymosins are the primary regulators of unpolymerized actin, and are essential for maintaining the small cytoplasmic pool of free G-actin monomers required for rapid filament elongation and allowing for the flux of monomers between the thymosin-bound pool and F-actin. Thymosin beta4 sequesters actin, holding it in a form that is unable to polymerize. Due to its profusion in the cytosol and its ability to bind ATP G-actin but not F-actin, thymosin beta4 is regarded as the principal actin-sequestering protein. Thymosin beta4 binds ATP G- monomeric actin in a 11 complex where G-actin cannot polymerize.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
thymosin beta-4
NCBI Official Synonym Full Names
thymosin beta 4 X-linked
NCBI Official Symbol
TMSB4X
NCBI Official Synonym Symbols
FX; TB4X; PTMB4; TMSB4
NCBI Protein Information
thymosin beta-4
UniProt Protein Name
Thymosin beta-4
Protein Family
UniProt Gene Name
TMSB4X
UniProt Synonym Gene Names
TB4X; THYB4; TMSB4; T beta-4
UniProt Entry Name
TYB4_HUMAN
NCBI Description
This gene encodes an actin sequestering protein which plays a role in regulation of actin polymerization. The protein is also involved in cell proliferation, migration, and differentiation. This gene escapes X inactivation and has a homolog on chromosome Y. [provided by RefSeq, Jul 2008]
Uniprot Description
TMSB4X: Plays an important role in the organization of the cytoskeleton. Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization. Belongs to the thymosin beta family.
Protein type: Motility/polarity/chemotaxis; Cytoskeletal
Chromosomal Location of Human Ortholog: Xq21.3-q22
Cellular Component: cytoskeleton; extracellular region
Molecular Function: actin monomer binding; protein binding
Biological Process: platelet activation; platelet degranulation; sequestering of actin monomers; actin filament organization; blood coagulation
Research Articles on TMSB4X
Similar Products
Product Notes
The Human TMSB4X tmsb4x (Catalog #AAA9713917) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713917 ELISA Kit recognizes Human TMSB4X. It is sometimes possible for the material contained within the vial of "Thymosin beta-4 (TMSB4X), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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