Human Thymidine Phosphorylase (TP) ELISA Kit | TP elisa kit

Human Thymidine Phosphorylase (TP) ELISA Kit

Cat. Num. AAA262471

• Gene Names: TYMP; TP; ECGF; ECGF1; MNGIE; MEDPS1; MTDPS1; PDECGF; hPD-ECGF
• Reactivity: Human
• Synonyms: Thymidine Phosphorylase (TP); Human Thymidine Phosphorylase (TP) ELISA Kit; TP elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 482

• Samples: Human serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: Up to 0.06 ng/ml
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TP elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human TP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 384229049
• NCBI GeneID: 1890
• NCBI Accession #: NP_001244917.1
• NCBI GenBank Nucleotide #: NM_001257988.1
• Molecular Weight: 50,405 Da
• NCBI Official Full Name: thymidine phosphorylase isoform 1 proprotein
• NCBI Official Synonym Full Names: thymidine phosphorylase
• NCBI Official Symbol: TYMP
• NCBI Official Synonym Symbols: TP; ECGF; ECGF1; MNGIE; MEDPS1; MTDPS1; PDECGF; hPD-ECGF
• NCBI Protein Information: thymidine phosphorylase; tdRPase; gliostatin
• UniProt Protein Name: Thymidine phosphorylase
• Protein Family: Trehalose phosphorylase
• UniProt Gene Name: TYMP
• UniProt Synonym Gene Names: ECGF1; TP; PD-ECGF
• UniProt Entry Name: TYPH_HUMAN

NCBI Description

This gene encodes an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in this gene have been associated with mitochondrial neurogastrointestinal encephalomyopathy. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Apr 2012]

Uniprot Description

ECGF1: May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro. Homodimer. Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family.

Protein type: Cytokine; Transferase; Xenobiotic Metabolism - drug metabolism - other enzymes; Phosphorylase; EC 2.4.2.4; DNA replication; Nucleotide Metabolism - pyrimidine; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: cytosol

Molecular Function: thymidine phosphorylase activity; growth factor activity; phosphorylase activity; transferase activity, transferring pentosyl groups; pyrimidine-nucleoside phosphorylase activity; platelet-derived growth factor receptor binding

Biological Process: pyrimidine nucleoside catabolic process; mitochondrial genome maintenance; pyrimidine base metabolic process; pyrimidine nucleotide metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside salvage; angiogenesis; cell differentiation; DNA replication; chemotaxis

Disease: Mitochondrial Dna Depletion Syndrome 1 (mngie Type)