Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Tetraspanin-1 (TSPAN1) Standard Curve.)
Human Tetraspanin-1 (TSPAN1) ELISA Kit | TSPAN1 elisa kit
Human Tetraspanin-1 (TSPAN1) ELISA Kit
Gene Names
TSPAN1; NET1; TM4C; TM4SF
Reactivity
Human
Synonyms
Tetraspanin-1 (TSPAN1); Human Tetraspanin-1 (TSPAN1) ELISA Kit; TSPAN1; NET1; TM4C; TM4SF; OTTHUMP00000008900; tetraspan 1; TSPAN1 elisa kit
Reactivity
Human
Specificity
Human Tetraspanin-1 (TSPAN1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TSPAN1. No significant cross-reactivity or interference between Human TSPAN1 and analogues was observed.
Sequence Length
241
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Tetraspanin-1 (TSPAN1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Tetraspanin-1 (TSPAN1) Standard Curve.)
Related Product Information for TSPAN1 elisa kit
Description: This Human Tetraspanin-1 (TSPAN1) ELISA Kit employs a two-site sandwich ELISA to quantitate TSPAN1 in samples. An antibody specific for TSPAN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TSPAN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TSPAN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSPAN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TSPAN1 belongs to the large family of tetraspanins, so named because they contain 4 transmembrane domains. Tetraspanins are frequently expressed at the cell surface in association with each other and with other molecules, such as integrins, and they function to regulate cell adhesion, migration, proliferation, and differentiation. The deduced 242-amino acid protein contains 4 transmembrane domains and other features characteristic of tetraspanins, including conservation of charged residues in or near the transmembrane domains, similar to ion channels, and of 4 cysteines in the second extracellular domain. EST database analysis suggested that TSPAN2 is expressed in colon, endometrium, pancreas, normal and neoplastic prostate, and pregnant uterus.
Background: TSPAN1 belongs to the large family of tetraspanins, so named because they contain 4 transmembrane domains. Tetraspanins are frequently expressed at the cell surface in association with each other and with other molecules, such as integrins, and they function to regulate cell adhesion, migration, proliferation, and differentiation. The deduced 242-amino acid protein contains 4 transmembrane domains and other features characteristic of tetraspanins, including conservation of charged residues in or near the transmembrane domains, similar to ion channels, and of 4 cysteines in the second extracellular domain. EST database analysis suggested that TSPAN2 is expressed in colon, endometrium, pancreas, normal and neoplastic prostate, and pregnant uterus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tetraspanin-1
NCBI Official Synonym Full Names
tetraspanin 1
NCBI Official Symbol
TSPAN1
NCBI Official Synonym Symbols
NET1; TM4C; TM4SF
NCBI Protein Information
tetraspanin-1
UniProt Protein Name
Tetraspanin-1
UniProt Gene Name
TSPAN1
UniProt Synonym Gene Names
Tspan-1
UniProt Entry Name
TSN1_HUMAN
NCBI Description
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. [provided by RefSeq, Jul 2008]
Research Articles on TSPAN1
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Product Notes
The Human TSPAN1 tspan1 (Catalog #AAA9715975) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715975 ELISA Kit recognizes Human TSPAN1. It is sometimes possible for the material contained within the vial of "Tetraspanin-1 (TSPAN1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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