Typical Testing Data/Standard Curve (for reference only)
Human Tachykinin Receptor 1 (TACR1) ELISA Kit | TACR1 elisa kit
Human Tachykinin Receptor 1 (TACR1) ELISA Kit
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TACR1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TACR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TACR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TACR1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to a gene family of tachykinin receptors. These tachykinin receptors are characterized by interactions with G proteins and contain seven hydrophobic transmembrane regions. This gene encodes the receptor for the tachykinin substance P, also referred to as neurokinin 1. The encoded protein is also involved in the mediation of phosphatidylinositol metabolism of substance P. [provided by RefSeq, Sep 2008]
Uniprot Description
TACR1: This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P > substance K > neuromedin-K. Belongs to the G-protein coupled receptor 1 family.
Protein type: Membrane protein, integral; GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 2p12
Cellular Component: cell surface; integral to plasma membrane; dendrite; cytoplasm; plasma membrane
Molecular Function: protein binding; substance P receptor activity; tachykinin receptor activity
Biological Process: response to nicotine; tachykinin signaling pathway; positive regulation of leukocyte migration; response to morphine; positive regulation of saliva secretion; response to estradiol stimulus; behavioral response to pain; elevation of cytosolic calcium ion concentration; regulation of smooth muscle cell migration; positive regulation of lymphocyte proliferation; positive regulation of stress fiber formation; positive regulation of action potential; inflammatory response; response to electrical stimulus; angiotensin mediated drinking behavior; detection of abiotic stimulus; associative learning; positive regulation of synaptic transmission, cholinergic; regulation of smooth muscle cell proliferation; eating behavior; positive regulation of synaptic transmission, GABAergic; positive regulation of hormone secretion; positive regulation of vascular permeability; positive regulation of ossification; response to ethanol; long-term memory; smooth muscle contraction involved in micturition; response to heat; response to ozone; operant conditioning; positive regulation of vasoconstriction; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); sperm ejaculation; response to progesterone stimulus; acute inflammatory response; positive regulation of epithelial cell proliferation; positive regulation of blood pressure
Research Articles on TACR1
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Product Notes
The Human TACR1 tacr1 (Catalog #AAA2024212) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2024212 ELISA Kit recognizes Human TACR1. It is sometimes possible for the material contained within the vial of "Tachykinin Receptor 1 (TACR1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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