Human Tachykinin Receptor 1 (TACR1) ELISA Kit | TACR1 elisa kit

Human Tachykinin Receptor 1 (TACR1) ELISA Kit

Cat. Num. AAA2024212

• Gene Names: TACR1; SPR; NK1R; NKIR; TAC1R
• Reactivity: Human
• Synonyms: Tachykinin Receptor 1 (TACR1); Human Tachykinin Receptor 1 (TACR1) ELISA Kit; NK1R; SPR; NKIR; TAC1R; Neurokinin 1 Receptor; Substance P Receptor; TACR1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TACR1. No significant cross-reactivity or interference between TACR1 and analogues was observed.
• Sequence Length: 311

• Samples: Tissue homogenates, cell lysates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: <0.124ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TACR1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TACR1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TACR1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TACR1 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TACR1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TACR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TACR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TACR1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TACR1 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 38565061
• NCBI GeneID: 6869
• UniProt Accession #: P25103; A8K150
• Molecular Weight: 35,713 Da
• NCBI Official Full Name: tachykinin receptor 1
• NCBI Official Synonym Full Names: tachykinin receptor 1
• NCBI Official Symbol: TACR1
• NCBI Official Synonym Symbols: SPR; NK1R; NKIR; TAC1R
• NCBI Protein Information: substance-P receptor
• UniProt Protein Name: Substance-P receptor
• Protein Family: Substance-P receptor
• UniProt Gene Name: TACR1
• UniProt Synonym Gene Names: NK1R; TAC1R; SPR; NK-1R
• UniProt Entry Name: NK1R_HUMAN

NCBI Description

This gene belongs to a gene family of tachykinin receptors. These tachykinin receptors are characterized by interactions with G proteins and contain seven hydrophobic transmembrane regions. This gene encodes the receptor for the tachykinin substance P, also referred to as neurokinin 1. The encoded protein is also involved in the mediation of phosphatidylinositol metabolism of substance P. [provided by RefSeq, Sep 2008]

Uniprot Description

TACR1: This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P > substance K > neuromedin-K. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: cell surface; integral to plasma membrane; dendrite; cytoplasm; plasma membrane

Molecular Function: protein binding; substance P receptor activity; tachykinin receptor activity

Biological Process: response to nicotine; tachykinin signaling pathway; positive regulation of leukocyte migration; response to morphine; positive regulation of saliva secretion; response to estradiol stimulus; behavioral response to pain; elevation of cytosolic calcium ion concentration; regulation of smooth muscle cell migration; positive regulation of lymphocyte proliferation; positive regulation of stress fiber formation; positive regulation of action potential; inflammatory response; response to electrical stimulus; angiotensin mediated drinking behavior; detection of abiotic stimulus; associative learning; positive regulation of synaptic transmission, cholinergic; regulation of smooth muscle cell proliferation; eating behavior; positive regulation of synaptic transmission, GABAergic; positive regulation of hormone secretion; positive regulation of vascular permeability; positive regulation of ossification; response to ethanol; long-term memory; smooth muscle contraction involved in micturition; response to heat; response to ozone; operant conditioning; positive regulation of vasoconstriction; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); sperm ejaculation; response to progesterone stimulus; acute inflammatory response; positive regulation of epithelial cell proliferation; positive regulation of blood pressure