Human T-cell surface glycoprotein CD3 zeta chain (CD247) ELISA Kit | CD247 elisa kit

Human T-cell surface glycoprotein CD3 zeta chain (CD247) ELISA Kit

Cat. Num. AAA166681

• Gene Names: CD247; T3Z; CD3H; CD3Q; CD3Z; TCRZ; IMD25; CD3-ZETA
• Reactivity: Human
• Synonyms: T-cell surface glycoprotein CD3 zeta chain (CD247); Human T-cell surface glycoprotein CD3 zeta chain (CD247) ELISA Kit; CD247 elisa kit

• Reactivity: Human
• Sequence Length: 164

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.3ng/ml - 90ng/ml
• Sensitivity: 0.16ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD247 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of human T-cell Surface Glycoprotein CD3 Zeta Chain (also known as CD247) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human CD247 antibody. CD247 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human CD247 Antibody is added and binds to CD247 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CD247 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human CD247. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 37595565
• NCBI GeneID: 919
• NCBI Accession #: NP_932170.1
• NCBI GenBank Nucleotide #: NM_198053.2
• UniProt Accession #: P20963; Q5VX13; Q8TAX4; B1AK49; A46461
• Molecular Weight: – Da
• NCBI Official Full Name: T-cell surface glycoprotein CD3 zeta chain isoform 1
• NCBI Official Synonym Full Names: CD247 molecule
• NCBI Official Symbol: CD247
• NCBI Official Synonym Symbols: T3Z; CD3H; CD3Q; CD3Z; TCRZ; IMD25; CD3-ZETA
• NCBI Protein Information: T-cell surface glycoprotein CD3 zeta chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 zeta chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD247
• UniProt Synonym Gene Names: CD3Z; T3Z; TCRZ

NCBI Description

The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098, PubMed:7509083). CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (PubMed:7509083). Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN) ().