Human T-cell Surface Glycoprotein CD1a (CD1A) ELISA Kit | CD1A elisa kit

Human T-cell Surface Glycoprotein CD1a (CD1A) ELISA Kit

Cat. Num. AAA2801439

• Gene Names: CD1A; R4; T6; CD1; FCB6; HTA1
• Reactivity: Human
• Synonyms: T-cell Surface Glycoprotein CD1a (CD1A); Human T-cell Surface Glycoprotein CD1a (CD1A) ELISA Kit; CD1; FCB6; HTA1; R4; T6; CD1A antigen; a polypeptide; T-cell surface antigen T6/Leu-6; T-cell surface glycoprotein CD1a; cluster of differentiation 1 A; cortical thymocyte antigen CD1A; diff; CD1A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human CD1A. No significant cross-reactivity or interference between Human CD1A and analogues was observed.
• Sequence Length: 327

• Assay Type: Quantitative Sandwich
• Samples: Serum, Plasma, Other biological fluids
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C. Please refer to Instruction Manual.

Related Product Information for CD1A elisa kit

This assay employs a two-site sandwich ELISA to quantitate CD1A in samples. An antibody specific for CD1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Overview: CD1d is a member of the CD1 (cluster of differentiation 1) family of glycoproteins expressed on the surface of various human antigen-presenting cells. They are related to the class I MHC molecules, and are involved in the presentation of lipid antigens to T cells. It is the only member of the group 2 CD1 molecules. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail.CD1d presented lipid antigens activate a special class of T cells, known as Natural Killer T (NKT) cells. When activated, NKT cells rapidly produce Th1 and Th2 cytokines, typically represented by interferon-gamma and IL-4 production.

NCBI and Uniprot Product Information

• NCBI GI #: 288558852
• NCBI GeneID: 909
• UniProt Accession #: P06126
• NCBI Official Full Name: T-cell surface glycoprotein CD1a
• NCBI Official Synonym Full Names: CD1a molecule
• NCBI Official Symbol: CD1A
• NCBI Official Synonym Symbols: R4; T6; CD1; FCB6; HTA1
• NCBI Protein Information: T-cell surface glycoprotein CD1a
• UniProt Protein Name: T-cell surface glycoprotein CD1a
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD1A
• UniProt Synonym Gene Names: hTa1 thymocyte antigen

NCBI Description

This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene localizes to the plasma membrane and to recycling vesicles of the early endocytic system. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Uniprot Description

Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.