Human T-Cell Acute Lymphocytic Leukemia Protein 1 (TAL1) ELISA Kit | TAL1 elisa kit

Human T-Cell Acute Lymphocytic Leukemia Protein 1 (TAL1) ELISA Kit

Cat. Num. AAA9714128

• Gene Names: TAL1; SCL; TCL5; tal-1; bHLHa17
• Reactivity: Human
• Synonyms: T-Cell Acute Lymphocytic Leukemia Protein 1 (TAL1); Human T-Cell Acute Lymphocytic Leukemia Protein 1 (TAL1) ELISA Kit; TAL1; SCL; TCL5; bHLHa17; tal-1; OTTHUMP00000009563; tal-1 product; TAL1 elisa kit

• Reactivity: Human
• Specificity: Human T-cell acute lymphocytic leukemia protein 1 (TAL1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TAL1. No significant cross-reactivity or interference between Human TAL1 and analogues was observed.
• Sequence Length: 331

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human T-cell acute lymphocytic leukemia protein 1 (TAL1) Standard Curve.)

Related Product Information for TAL1 elisa kit

Description: This Human T-cell acute lymphocytic leukemia protein 1 (TAL1) ELISA Kit employs a two-site sandwich ELISA to quantitate TAL1 in samples. An antibody specific for TAL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TAL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: TAL1 (TAL BHLH Transcription Factor 1, Erythroid Differentiation Factor) is a Protein Coding gene. Diseases associated with TAL1 include Leukemia, Acute Lymphoblastic and Precursor T-Cell Acute Lymphoblastic Leukemia. Among its related pathways are MAPK-Erk Pathway and NF-kappaB Signaling. GO annotations related to this gene include transcription factor activity, sequence-specific DNA binding and chromatin binding. An important paralog of this gene is LYL1. It may play an important role in hemopoietic differentiation. Serves as a positive regulator of erythroid differentiation.

NCBI and Uniprot Product Information

• NCBI GI #: 4507363
• NCBI GeneID: 6886
• NCBI Accession #: NP_003180.1
• NCBI GenBank Nucleotide #: NP_003180.1
• UniProt Accession #: P17542
• NCBI Official Full Name: T-cell acute lymphocytic leukemia protein 1 isoform 1
• NCBI Official Synonym Full Names: TAL bHLH transcription factor 1, erythroid differentiation factor
• NCBI Official Symbol: TAL1
• NCBI Official Synonym Symbols: SCL; TCL5; tal-1; bHLHa17
• NCBI Protein Information: T-cell acute lymphocytic leukemia protein 1
• UniProt Protein Name: T-cell acute lymphocytic leukemia protein 1
• Protein Family: Transaldolase
• UniProt Gene Name: TAL1
• UniProt Synonym Gene Names: BHLHA17; SCL; TCL5; TAL-1; bHLHa17
• UniProt Entry Name: TAL1_HUMAN

Uniprot Description

TAL1: a basic helix-loop-helix transcription. Regulates differentiation and survival during hemopoiesis. Implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation. Serves as a positive regulator of eryhtroid differentiation. Mutations are associated with T-cell leukemia and melanoma. Binds to the LIM domain containing protein Rhombotin-2.

Protein type: DNA-binding; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 1p32

Cellular Component: transcription factor complex; histone deacetylase complex; nuclear chromatin; nucleus

Molecular Function: protein binding; enzyme binding; protein heterodimerization activity; histone deacetylase binding; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; erythrocyte maturation; cell fate commitment; positive regulation of chromatin assembly or disassembly; embryonic hemopoiesis; megakaryocyte differentiation; positive regulation of transcription, DNA-dependent; positive regulation of erythrocyte differentiation; astrocyte fate commitment; locomotory behavior; negative regulation of transcription from RNA polymerase II promoter; positive regulation of mitotic cell cycle; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; positive regulation of protein complex assembly; positive regulation of cell division; erythrocyte differentiation; spinal cord association neuron differentiation; hemopoiesis; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; platelet formation; basophil differentiation

Disease: Leukemia, Acute Lymphoblastic