Human T-Box Protein 21 (TBX21) ELISA Kit | TBX21 elisa kit

Human T-Box Protein 21 (TBX21) ELISA Kit

Cat. Num. AAA2707181

• Gene Names: TBX21; TBET; T-PET; T-bet; TBLYM
• Reactivity: Human
• Synonyms: T-Box Protein 21 (TBX21); Human T-Box Protein 21 (TBX21) ELISA Kit; TBLYM; TBET; T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet; TBX21 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of T-Box Protein 21 (TBX21).; No significant cross-reactivity or interference between T-Box Protein 21 (TBX21) and analogues was observed.
• Sequence Length: 535

• Assay Type: Double-antibody Sandwich
• Samples: Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.052ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level T-Box Protein 21 (TBX21) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level T-Box Protein 21 (TBX21) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TBX21 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-Box Protein 21 (TBX21). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to T-Box Protein 21 (TBX21). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain T-Box Protein 21 (TBX21), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of T-Box Protein 21 (TBX21) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 7019549
• NCBI GeneID: 30009
• NCBI Accession #: NP_037483.1
• NCBI GenBank Nucleotide #: NM_013351.1
• UniProt Accession #: Q9UL17
• Molecular Weight: 58,328 Da
• NCBI Official Full Name: T-box transcription factor TBX21
• NCBI Official Synonym Full Names: T-box 21
• NCBI Official Symbol: TBX21
• NCBI Official Synonym Symbols: TBET; T-PET; T-bet; TBLYM
• NCBI Protein Information: T-box transcription factor TBX21
• UniProt Protein Name: T-box transcription factor TBX21
• Protein Family: T-box transcription factor
• UniProt Gene Name: TBX21
• UniProt Synonym Gene Names: TBET; TBLYM; T-box protein 21

NCBI Description

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells. [provided by RefSeq, Jul 2008]

Uniprot Description

TBX21: Transcription factor that controls the expression of the TH1 cytokine, interferon-gamma. Initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. T-cell specific.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: cell soma; nucleus

Molecular Function: DNA binding transcription factor activity; protein binding; sequence-specific DNA binding; transcription regulatory region DNA binding

Biological Process: cellular response to organic substance; lymphocyte migration; multicellular organism development; negative regulation of interleukin-2 production; negative regulation of T-helper 17 cell differentiation; negative regulation of T-helper 17 cell lineage commitment; negative regulation of T-helper 2 cell cytokine production; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of isotype switching to IgG isotypes; positive regulation of transcription, DNA-templated; proteasomal ubiquitin-dependent protein catabolic process; regulation of T cell differentiation; regulation of transcription, DNA-templated; response to virus; T cell differentiation; transcription, DNA-dependent

Disease: Asthma, Nasal Polyps, And Aspirin Intolerance