Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Nesprin-1 (SYNE1) Standard Curve.)
Mouse Nesprin-1 (SYNE1) ELISA Kit | SYNE1 elisa kit
Mouse Nesprin-1 (SYNE1) ELISA Kit
Gene Names
Syne1; 8B; CPG2; KASH1; Myne1; myne-1; BE692247; mKIAA1756; A330049M09Rik; C130039F11Rik
Reactivity
Mouse
Synonyms
Nesprin-1 (SYNE1); Mouse Nesprin-1 (SYNE1) ELISA Kit; RP1-130E4.2; 8B; C6orf98; CPG2; DKFZp781J13156; EDMD4; FLJ30878; FLJ41140; KIAA0796; KIAA1262; KIAA1756; MYNE1; SCAR8; dJ45H2.2; OTTHUMP00000017438; enaptin; myocyte nuclear envelope protein 1; nesprin; SYNE1 elisa kit
Reactivity
Mouse
Specificity
Mouse Nesprin-1 (SYNE1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SYNE1. No significant cross-reactivity or interference between Mouse SYNE1 and analogues was observed.
Sequence Length
3010
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Nesprin-1 (SYNE1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Nesprin-1 (SYNE1) Standard Curve.)
Related Product Information for SYNE1 elisa kit
Description: This Mouse Nesprin-1 (SYNE1) ELISA Kit employs a two-site sandwich ELISA to quantitate SYNE1 in samples. An antibody specific for SYNE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SYNE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYNE1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYNE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Enaptin is a nuclear envelope protein found in human myocytes and synapses, which is made up of 8,797 amino acids. Post-translational amino acid modifications can be possible. Enaptin is involved in the maintenance of nuclear organization and structural integrity, tethering the cell nucleus to the cytoskeleton by interacting with the nuclear envelope and with F-actin in the cytoplasm. Enaptin has a remarkable structure with a coiled alpha-helical region and a large beta-sheet region in the upper part and at least four alpha-helices spliced together, indicating the similarity with collagen. The structural integrity of Enaptin can be shown by determining whether the protein forms a condensed supramolecule.
Background: Enaptin is a nuclear envelope protein found in human myocytes and synapses, which is made up of 8,797 amino acids. Post-translational amino acid modifications can be possible. Enaptin is involved in the maintenance of nuclear organization and structural integrity, tethering the cell nucleus to the cytoskeleton by interacting with the nuclear envelope and with F-actin in the cytoplasm. Enaptin has a remarkable structure with a coiled alpha-helical region and a large beta-sheet region in the upper part and at least four alpha-helices spliced together, indicating the similarity with collagen. The structural integrity of Enaptin can be shown by determining whether the protein forms a condensed supramolecule.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
nesprin-1 isoform 3
NCBI Official Synonym Full Names
spectrin repeat containing, nuclear envelope 1
NCBI Official Symbol
Syne1
NCBI Official Synonym Symbols
8B; CPG2; KASH1; Myne1; myne-1; BE692247; mKIAA1756; A330049M09Rik; C130039F11Rik
NCBI Protein Information
nesprin-1
UniProt Protein Name
Nesprin-1
Protein Family
UniProt Gene Name
Syne1
UniProt Synonym Gene Names
Myne-1; Syne-1
UniProt Entry Name
SYNE1_MOUSE
Research Articles on SYNE1
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Product Notes
The Mouse SYNE1 syne1 (Catalog #AAA9716895) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716895 ELISA Kit recognizes Mouse SYNE1. It is sometimes possible for the material contained within the vial of "Nesprin-1 (SYNE1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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