Typical Testing Data/Standard Curve (for reference only)
Human Survival Motor Neuron Domain Containing Protein 1 (SMNDC1) ELISA Kit | SMNDC1 elisa kit
Human Survival Motor Neuron Domain Containing Protein 1 (SMNDC1) ELISA Kit
Gene Names
SMNDC1; SMNR; SPF30; TDRD16C
Reactivity
Human
Synonyms
Survival Motor Neuron Domain Containing Protein 1 (SMNDC1); Human Survival Motor Neuron Domain Containing Protein 1 (SMNDC1) ELISA Kit; SMNR; SPF30; Survival Of Motor Neuron Related Splicing Factor 30; 30 kDa splicing factor SMNrp; SMN-related protein; SMNDC1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Survival Motor Neuron Domain Containing Protein 1 (SMNDC1).
No significant cross-reactivity or interference between Survival Motor Neuron Domain Containing Protein 1 (SMNDC1) and analogues was observed.
No significant cross-reactivity or interference between Survival Motor Neuron Domain Containing Protein 1 (SMNDC1) and analogues was observed.
Sequence Length
238
Assay Type
Double-antibody Sandwich
Sample
Tissue Homogenates, Cell Lysates and Other Biological Fluids
Dectection Range
0.156-10ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.056ng/mL
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Survival Motor Neuron Domain Containing Protein 1 (SMNDC1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Survival Motor Neuron Domain Containing Protein 1 (SMNDC1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Survival Motor Neuron Domain Containing Protein 1 (SMNDC1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SMNDC1 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Survival Motor Neuron Domain Containing Protein 1 (SMNDC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Survival Motor Neuron Domain Containing Protein 1 (SMNDC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Survival Motor Neuron Domain Containing Protein 1 (SMNDC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Survival Motor Neuron Domain Containing Protein 1 (SMNDC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for SMNDC1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Survival of motor neuron-related-splicing factor 30
NCBI Official Synonym Full Names
survival motor neuron domain containing 1
NCBI Official Symbol
SMNDC1
NCBI Official Synonym Symbols
SMNR; SPF30; TDRD16C
NCBI Protein Information
survival of motor neuron-related-splicing factor 30
UniProt Protein Name
Survival of motor neuron-related-splicing factor 30
Protein Family
UniProt Gene Name
SMNDC1
UniProt Synonym Gene Names
SMNR; SPF30
UniProt Entry Name
SPF30_HUMAN
NCBI Description
This gene is a paralog of SMN1 gene, which encodes the survival motor neuron protein, mutations in which are cause of autosomal recessive proximal spinal muscular atrophy. The protein encoded by this gene is a nuclear protein that has been identified as a constituent of the spliceosome complex. This gene is differentially expressed, with abundant levels in skeletal muscle, and may share similar cellular function as the SMN1 gene. [provided by RefSeq, Jul 2008]
Uniprot Description
SPF30: Necessary for spliceosome assembly. Overexpression causes apoptosis. Belongs to the SMN family.
Protein type: Spliceosome; RNA processing; RNA splicing; RNA-binding
Chromosomal Location of Human Ortholog: 10q23
Cellular Component: nucleoplasm; spliceosome; Cajal body; intermediate filament cytoskeleton; cytoplasm; nuclear speck; nucleus
Molecular Function: protein binding
Biological Process: RNA splicing, via transesterification reactions; apoptosis; RNA splicing; spliceosome assembly; mRNA processing
Research Articles on SMNDC1
Similar Products
Product Notes
The Human SMNDC1 smndc1 (Catalog #AAA2122175) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2122175 ELISA Kit recognizes Human SMNDC1. It is sometimes possible for the material contained within the vial of "Survival Motor Neuron Domain Containing Protein 1 (SMNDC1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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