Human Spondin 2 (SPON2) ELISA Kit | SPON2 elisa kit

Human Spondin 2 (SPON2) ELISA Kit

Cat. Num. AAA452752

• Gene Names: SPON2; DIL1; DIL-1; MINDIN; M-SPONDIN
• Reactivity: Human
• Synonyms: Spondin 2 (SPON2); Human Spondin 2 (SPON2) ELISA Kit; DIL1 Mindin M-spondin Extracellular Matrix Protein Differentially expressed in cancerous and non-cancerous lung cells 1; SPON2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SPON2. No significant cross-reactivity or interference between SPON2 and analogues was observed.
• Sequence Length: 331

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 62.5-4000pg/mL
• Sensitivity: 27.6pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SPON2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SPON2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Standard Curve/Testing Data

Related Product Information for SPON2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SPON2 in human serum, plasma, tissue homogenates, and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an `antibody specific to SPON2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SPON2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SPON2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SPON2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 190341087
• NCBI GeneID: 10417
• NCBI Accession #: NP_001121797.1
• NCBI GenBank Nucleotide #: NM_001128325.2
• UniProt Accession #: Q9BUD6; Q4W5N4; Q9ULW1; D3DVN9
• Molecular Weight: 35,846 Da
• NCBI Official Full Name: spondin-2
• NCBI Official Synonym Full Names: spondin 2
• NCBI Official Symbol: SPON2
• NCBI Official Synonym Symbols: DIL1; DIL-1; MINDIN; M-SPONDIN
• NCBI Protein Information: spondin-2
• UniProt Protein Name: Spondin-2
• Protein Family: Spondin
• UniProt Gene Name: SPON2
• UniProt Synonym Gene Names: DIL1; DIL-1

Uniprot Description

Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens (). Binds bacterial lipopolysaccharide (LPS).