Human Spondin-1 (SPON1) ELISA Kit | SPON1 elisa kit

Human Spondin-1 (SPON1) ELISA Kit

Cat. Num. AAA2801609

• Gene Names: SPON1; f-spondin; VSGP/F-spondin
• Reactivity: Human
• Synonyms: Spondin-1 (SPON1); Human Spondin-1 (SPON1) ELISA Kit; KIAA0762; MGC10724; f-spondin; VSGP/F-spondin; spondin 1; (f-spondin) extracellular matrix protein; SPON1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human SPON1. No significant cross-reactivity or interference between Human SPON1 and analogues was observed.

• Asssay Type: Quantitive Sandwich
• Sample Types: Serum, Plasma, Other biological fluids
• Detection Wavelength: 450nm
• Sample Volume: 1-200uL
• Precision: Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV<8%; Inter-Assay: CV<12%
• Test Principle: This assay employs a two-site sandwich ELISA to quantitate SPON1 in samples. An antibody specific for SPON1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPON1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPON1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPON1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Assay Duration: 1-4.5h
• Preparation and Storage: Store at 2-8 degree C

NCBI and Uniprot Product Information

• NCBI GI #: 110347423
• NCBI GeneID: 10418
• NCBI Accession #: NP_006099.2
• NCBI GenBank Nucleotide #: NM_006108.3
• UniProt Accession #: Q9HCB6; O94862; Q8NCD7; Q8WUR5; A8K6W5
• Molecular Weight: 90,973 Da
• NCBI Official Full Name: spondin-1
• NCBI Official Synonym Full Names: spondin 1, extracellular matrix protein
• NCBI Official Symbol: SPON1
• NCBI Official Synonym Symbols: f-spondin; VSGP/F-spondin
• NCBI Protein Information: spondin-1; spondin 1, (f-spondin) extracellular matrix protein; vascular smooth muscle cell growth-promoting factor
• UniProt Protein Name: Spondin-1
• Protein Family: Spondin
• UniProt Gene Name: SPON1
• UniProt Synonym Gene Names: KIAA0762; VSGP

Uniprot Description

Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS (). Major factor for vascular smooth muscle cell.