Human Sortilin-related receptor (SORL1) ELISA Kit | SORL1 elisa kit

Human Sortilin-related receptor (SORL1) ELISA Kit

Cat. Num. AAA2802029

• Gene Names: SORL1; LR11; LRP9; SORLA; gp250; SorLA-1; C11orf32
• Reactivity: Human
• Synonyms: Sortilin-related receptor (SORL1); Human Sortilin-related receptor (SORL1) ELISA Kit; C11orf32; FLJ21930; FLJ39258; LR11; LRP9; SORLA; SorLA-1; gp250; mosaic protein LR11|sortilin-related receptor containing LDLR class A repeats; SORL1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human SORL1. No significant cross-reactivity or interference between Human SORL1 and analogues was observed.

• Assay Type: Sandwich
• Assay: Serum, Plasma, Other biological fluids
• Analysis Method: Quantitive
• Detection Wavelength: 450nm
• Sample Volume: 1-200uL
• Assay Duration: 1-4.5h
• Precision: Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Test Principle: This assay employs a two-site sandwich ELISA to quantitate SORL1 in samples. An antibody specific for SORL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySORL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SORL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SORL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
• Preparation and Storage: Store at 2-8 degree C. Please refer to Instruction Manual.

NCBI and Uniprot Product Information

• NCBI GI #: 4507157
• NCBI GeneID: 6653
• NCBI Accession #: NP_003096.1
• NCBI GenBank Nucleotide #: NM_003105.5
• UniProt Accession #: Q92673; Q92856; B2RNX7
• Molecular Weight: 248,426 Da
• NCBI Official Full Name: sortilin-related receptor preproprotein
• NCBI Official Synonym Full Names: sortilin-related receptor, L(DLR class) A repeats containing
• NCBI Official Symbol: SORL1
• NCBI Official Synonym Symbols: LR11; LRP9; SORLA; gp250; SorLA-1; C11orf32
• NCBI Protein Information: sortilin-related receptor; mosaic protein LR11; LDLR relative with 11 ligand-binding repeats; sortilin-related receptor, L(DLR class) A repeats-containing; sorting protein-related receptor containing LDLR class A repeats; low-density lipoprotein receptor
• UniProt Protein Name: Sortilin-related receptor
• Protein Family: Sortilin-related receptor
• UniProt Gene Name: SORL1
• UniProt Synonym Gene Names: C11orf32; LDLR relative with 11 ligand-binding repeats; LR11; SorLA
• UniProt Entry Name: SORL_HUMAN

NCBI Description

This gene encodes a mosaic protein that belongs to at least two families: the vacuolar protein sorting 10 (VPS10) domain-containing receptor family, and the low density lipoprotein receptor (LDLR) family. The encoded protein also contains fibronectin type III repeats and an epidermal growth factor repeat. The encoded protein is translated as a preproprotein and likely plays roles in endocytosis and sorting. There may be an association between expression of this locus and Alzheimer's Disease.[provided by RefSeq, Sep 2010]

Uniprot Description

SORL1: Likely to be a multifunctional endocytic receptor, that may be implicated in the uptake of lipoproteins and of proteases. Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. Binds the receptor- associated protein (RAP). Could play a role in cell-cell interaction. Belongs to the VPS10-related sortilin family. SORL1 subfamily.

Protein type: Cell surface; Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q23.2-q24.2

Cellular Component: Golgi apparatus; extracellular space; Golgi cisterna; integral to plasma membrane; endoplasmic reticulum; early endosome; trans-Golgi network; multivesicular body; recycling endosome; cell soma; membrane; nuclear envelope lumen; endosome

Molecular Function: ADP-ribosylation factor binding; protein binding; transmembrane receptor activity; beta-amyloid binding; low-density lipoprotein binding

Biological Process: cholesterol metabolic process; negative regulation of MAP kinase activity; receptor-mediated endocytosis; protein targeting to lysosome; cell migration; protein maturation; lipid transport; signal transduction; negative regulation of neurogenesis; negative regulation of protein oligomerization; cell proliferation; regulation of smooth muscle cell migration; positive regulation of protein catabolic process; post-Golgi vesicle-mediated transport; protein targeting to Golgi; negative regulation of protein binding; protein retention in Golgi; protein targeting

Disease: Alzheimer Disease