Human Solute Carrier Family 16, Member 1 (SLC16A1) ELISA Kit | SLC16A1 elisa kit

Human Solute Carrier Family 16, Member 1 (SLC16A1) ELISA Kit

Cat. Num. AAA455663

• Gene Names: SLC16A1; MCT; HHF7; MCT1; MCT1D
• Reactivity: Human
• Synonyms: Solute Carrier Family 16; Member 1 (SLC16A1); Human Solute Carrier Family 16; Member 1 (SLC16A1) ELISA Kit; MCT1; MCT; Monocarboxylate Transporter 1; Monocarboxylic Acid Transporter 1; SLC16A1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SLC16A1. No significant cross-reactivity or interference between SLC16A1 and analogues was observed.
• Sequence Length: 500

• Samples: Human tissue homogenates, cell lysates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.116ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SLC16A1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SLC16A1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SLC16A1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SLC16A1 in human tissue homogenates, cell lysates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SLC16A1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SLC16A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SLC16A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SLC16A1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 262073007
• NCBI GeneID: 6566
• NCBI Accession #: NP_001159968.1
• NCBI GenBank Nucleotide #: NM_001166496.1
• UniProt Accession #: P53985; Q49A45; Q5T8R6; Q9NSJ9
• Molecular Weight: 46,234 Da
• NCBI Official Full Name: monocarboxylate transporter 1
• NCBI Official Synonym Full Names: solute carrier family 16 member 1
• NCBI Official Symbol: SLC16A1
• NCBI Official Synonym Symbols: MCT; HHF7; MCT1; MCT1D
• NCBI Protein Information: monocarboxylate transporter 1
• UniProt Protein Name: Monocarboxylate transporter 1
• Protein Family: Monocarboxylate transporter
• UniProt Gene Name: SLC16A1
• UniProt Synonym Gene Names: MCT1; MCT 1

NCBI Description

The protein encoded by this gene is a proton-linked monocarboxylate transporter that catalyzes the movement of many monocarboxylates, such as lactate and pyruvate, across the plasma membrane. Mutations in this gene are associated with erythrocyte lactate transporter defect. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Oct 2009]

Uniprot Description

Proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Depending on the tissue and on cicumstances, mediates the import or export of lactic acid and ketone bodies. Required for normal nutrient assimilation, increase of white adipose tissue and body weight gain when on a high-fat diet. Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate, small molecules that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis.