Human Snail Homolog 1 (SNAI1) ELISA Kit | SNAI1 elisa kit

Human Snail Homolog 1 (SNAI1) ELISA Kit

Cat. Num. AAA450562

• Gene Names: SNAI1; SNA; SNAH; SNAIL; SLUGH2; SNAIL1; dJ710H13.1
• Reactivity: Human
• Synonyms: Snail Homolog 1 (SNAI1); Human Snail Homolog 1 (SNAI1) ELISA Kit; SLUGH2; SNA; SNAH; Snail 1 Zinc Finger Protein; SNAI1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SNAI1. No significant cross-reactivity or interference between SNAI1 and analogues was observed.
• Sequence Length: 264

• Samples: Human tissue homogenates, cell lysates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SNAI1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SNAI1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SNAI1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SNAI1 in human tissue homogenates, cell lysates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SNAI1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SNAI1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SNAI1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SNAI1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 18765741
• NCBI GeneID: 6615
• NCBI Accession #: NP_005976.2
• NCBI GenBank Nucleotide #: NM_005985.3
• UniProt Accession #: O95863; Q9P113; Q9UBP7; Q9UHH7; B2R842
• Molecular Weight: 29,083 Da
• NCBI Official Full Name: zinc finger protein SNAI1
• NCBI Official Synonym Full Names: snail family transcriptional repressor 1
• NCBI Official Symbol: SNAI1
• NCBI Official Synonym Symbols: SNA; SNAH; SNAIL; SLUGH2; SNAIL1; dJ710H13.1
• NCBI Protein Information: zinc finger protein SNAI1
• UniProt Protein Name: Zinc finger protein SNAI1
• Protein Family: Protein snail
• UniProt Gene Name: SNAI1
• UniProt Synonym Gene Names: SNAH; Protein sna

NCBI Description

The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself.