Human sideroflexin 4 ELISA Kit | SFXN4 elisa kit

Human Sideroflexin-4, SFXN4 ELISA Kit

Cat. Num. AAA2602784

• Gene Names: SFXN4; BCRM1; COXPD18
• Reactivity: Human
• Synonyms: sideroflexin 4; Human Sideroflexin-4; SFXN4 ELISA Kit; SFXN4 elisa kit

• Reactivity: Human

• Samples: Human serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 10 ng/mL-0.156 ng/mL
• Sensitivity: Up to 0.05 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SFXN4 elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human SFXN4 monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 47458811
• NCBI GeneID: 119559
• NCBI Accession #: NP_998814.1
• NCBI GenBank Nucleotide #: NM_213649.1
• UniProt Accession #: Q6P4A7; Q6WSU4; Q86TD9
• Molecular Weight: 37,998 Da
• NCBI Official Full Name: sideroflexin-4
• NCBI Official Synonym Full Names: sideroflexin 4
• NCBI Official Symbol: SFXN4
• NCBI Official Synonym Symbols: BCRM1; COXPD18
• NCBI Protein Information: sideroflexin-4; breast cancer resistance marker 1
• UniProt Protein Name: Sideroflexin-4
• Protein Family: Sideroflexin
• UniProt Gene Name: SFXN4
• UniProt Synonym Gene Names: BCRM1
• UniProt Entry Name: SFXN4_HUMAN

NCBI Description

This gene encodes a member of the sideroflexin family. The encoded protein is a transmembrane protein of the inner mitochondrial membrane, and is required for mitochondrial respiratory homeostasis and erythropoiesis. Mutations in this gene are associated with mitochondriopathy and macrocytic anemia. Alternatively spliced transcript variants have been found in this gene. [provided by RefSeq, Jan 2014]

Uniprot Description

SFXN4: Potential iron transporter. Belongs to the sideroflexin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 10q26.11

Cellular Component: intracellular membrane-bound organelle; mitochondrial inner membrane; integral to membrane

Molecular Function: ion transmembrane transporter activity

Biological Process: iron ion homeostasis

Disease: Combined Oxidative Phosphorylation Deficiency 18