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Human Sialomucin core protein 24 (CD164) ELISA Kit | CD164 elisa kit

Human Sialomucin core protein 24 (CD164) ELISA Kit

Gene Names
CD164; DFNA66; MGC-24; MUC-24; MGC-24v; endolyn
Reactivity
Human
Synonyms
Sialomucin core protein 24 (CD164); Human Sialomucin core protein 24 (CD164) ELISA Kit; RP11-425D10.9; MGC-24; MUC-24; endolyn; CD164 antigen; sialomucin; CD164 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human CD164. No significant cross-reactivity or interference between Human CD164 and analogues was observed.
Assay Type
Sandwich
Assay
Serum, Plasma, Other biological fluids
Analysis Method
Quantitive
Detection Wavelength
450nm
Sample Volume
1-200uL
Assay Duration
1-4.5h
Precision
Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Test Principle
This assay employs a two-site sandwich ELISA to quantitate CD164 in samples. An antibody specific for CD164 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCD164 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD164 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD164 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Preparation and Storage
Store at 2-8 degree C. Please refer to Instruction Manual.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
16,736 Da
NCBI Official Full Name
Sialomucin core protein 24
NCBI Official Synonym Full Names
CD164 molecule
NCBI Official Symbol
CD164
NCBI Official Synonym Symbols
DFNA66; MGC-24; MUC-24; MGC-24v; endolyn
NCBI Protein Information
sialomucin core protein 24
UniProt Protein Name
Sialomucin core protein 24
Protein Family
UniProt Gene Name
CD164
UniProt Synonym Gene Names
MUC-24; MGC-24; MGC-24v

NCBI Description

This gene encodes a transmembrane sialomucin and cell adhesion molecule that regulates the proliferation, adhesion and migration of hematopoietic progenitor cells. The encoded protein also interacts with the C-X-C chemokine receptor type 4 and may regulate muscle development. Elevated expression of this gene has been observed in human patients with Sezary syndrome, a type of blood cancer, and a mutation in this gene may be associated with impaired hearing. [provided by RefSeq, Oct 2016]

Uniprot Description

Sialomucin that may play a key role in hematopoiesis by facilitating the adhesion of CD34+ cells to the stroma and by negatively regulating CD34+CD38(lo/-) cell proliferation. Modulates the migration of umbilical cord blood CD133+ cells and this is mediated through the CXCL12/CXCR4 axis. May play an important role in prostate cancer metastasis and the infiltration of bone marrow by cancer cells. Promotes myogenesis by enhancing CXCR4-dependent cell motility. Positively regulates myoblast migration and promotes myoblast fusion into myotubes ().

Research Articles on CD164

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Product Notes

The Human CD164 cd164 (Catalog #AAA2802263) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2802263 ELISA Kit recognizes Human CD164. It is sometimes possible for the material contained within the vial of "Sialomucin core protein 24 (CD164), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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