Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Sialic acid-binding Ig-like lectin 6 (SIGLEC6) Standard Curve.)
Human Sialic Acid-Binding Ig-Like Lectin 6 (SIGLEC6) ELISA Kit | SIGLEC6 elisa kit
Human Sialic Acid-Binding Ig-Like Lectin 6 (SIGLEC6) ELISA Kit
Gene Names
SIGLEC6; CD327; CD33L; OBBP1; CD33L1; CD33L2; CDW327
Reactivity
Human
Synonyms
Sialic Acid-Binding Ig-Like Lectin 6 (SIGLEC6); Human Sialic Acid-Binding Ig-Like Lectin 6 (SIGLEC6) ELISA Kit; SIGLEC6; CD327; CD33L; CD33L1; CDw327; OBBP1; SIGLEC-6; CD33 antigen-like 1; CDw327 antigen; Obesity-binding protein 1; SIGLEC6 elisa kit
Reactivity
Human
Specificity
Human Sialic acid-binding Ig-like lectin 6 (SIGLEC6) ELISA Kit has high sensitivity and excellent specificity for detection of Human SIGLEC6. No significant cross-reactivity or interference between Human SIGLEC6 and analogues was observed.
Sequence Length
453
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Sialic acid-binding Ig-like lectin 6 (SIGLEC6) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Sialic acid-binding Ig-like lectin 6 (SIGLEC6) Standard Curve.)
Related Product Information for SIGLEC6 elisa kit
Description: This Human Sialic acid-binding Ig-like lectin 6 (SIGLEC6) ELISA Kit employs a two-site sandwich ELISA to quantitate SIGLEC6 in samples. An antibody specific for SIGLEC6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SIGLEC6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIGLEC6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIGLEC6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The deduced 442-amino acid CD33L protein contains a signal peptide, an N-terminal Ig-like V-domain, and 2 adjacent Ig C2-like domains, followed by a transmembrane region and a cytoplasmic tail. Based on its predicted structure, the authors stated that CD33L belongs to the Ig superfamily and is likely a novel member of the sialoadhesin subfamily. Compared to the original cDNA, this cDNA contains a 176-bp deletion in the coding sequence, resulting in a predicted 342-amino acid protein lacking the transmembrane and cytoplasmic regions. By RT-PCR of placenta RNA, the authors detected both transcripts, although the transcript encoding the membrane-bound isoform, CD33L1, was considerably more abundant.CD33L expression only in the placenta; transcripts of 4 distinct sizes were found, including 1 that was differentially polyadenylated.
Background: The deduced 442-amino acid CD33L protein contains a signal peptide, an N-terminal Ig-like V-domain, and 2 adjacent Ig C2-like domains, followed by a transmembrane region and a cytoplasmic tail. Based on its predicted structure, the authors stated that CD33L belongs to the Ig superfamily and is likely a novel member of the sialoadhesin subfamily. Compared to the original cDNA, this cDNA contains a 176-bp deletion in the coding sequence, resulting in a predicted 342-amino acid protein lacking the transmembrane and cytoplasmic regions. By RT-PCR of placenta RNA, the authors detected both transcripts, although the transcript encoding the membrane-bound isoform, CD33L1, was considerably more abundant.CD33L expression only in the placenta; transcripts of 4 distinct sizes were found, including 1 that was differentially polyadenylated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Sialic acid-binding Ig-like lectin 6
NCBI Official Synonym Full Names
sialic acid binding Ig like lectin 6
NCBI Official Symbol
SIGLEC6
NCBI Official Synonym Symbols
CD327; CD33L; OBBP1; CD33L1; CD33L2; CDW327
NCBI Protein Information
sialic acid-binding Ig-like lectin 6
UniProt Protein Name
Sialic acid-binding Ig-like lectin 6
Protein Family
UniProt Gene Name
SIGLEC6
UniProt Synonym Gene Names
CD33L; CD33L1; OBBP1; Siglec-6; OB-BP1
NCBI Description
This gene encodes a member of the SIGLEC (sialic acid binding immunoglobulin-like lectin) family of proteins. The encoded transmembrane receptor binds sialyl-TN glycans and leptin. Placental expression of the encoded protein is upregulated in preeclampsia. [provided by RefSeq, Jul 2016]
Uniprot Description
Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
Research Articles on SIGLEC6
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Product Notes
The Human SIGLEC6 siglec6 (Catalog #AAA9714326) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714326 ELISA Kit recognizes Human SIGLEC6. It is sometimes possible for the material contained within the vial of "Sialic Acid-Binding Ig-Like Lectin 6 (SIGLEC6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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