Human Shugoshin-like 1, SGOL1 ELISA Kit | SGOL1 elisa kit

Human Shugoshin-like 1, SGOL1 ELISA Kit

Cat. Num. AAA2602713

• Gene Names: SGOL1; SGO; Sgo1; NY-BR-85
• Reactivity: Human
• Synonyms: Shugoshin-like 1; SGOL1; Human Shugoshin-like 1; SGOL1 ELISA Kit; SGOL1 elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.

• Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant SGOL1 concentration
• Assay Type: Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: 0.06 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SGOL1 elisa kit

Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human SGOL1 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 60302875
• NCBI GeneID: 151648
• NCBI Accession #: NP_001012409.1
• NCBI GenBank Nucleotide #: NM_001012409.2
• UniProt Accession #: Q5FBB7; Q588H5; Q5FBB4; Q5FBB5; Q5FBB6; Q5FBB8; Q8N579; Q8WVL0; Q9BVA8; Q9H275
• Molecular Weight: 64,190 Da
• NCBI Official Full Name: shugoshin-like 1 isoform A1
• NCBI Official Synonym Full Names: shugoshin-like 1 (S. pombe)
• NCBI Official Symbol: SGOL1
• NCBI Official Synonym Symbols: SGO; Sgo1; NY-BR-85
• NCBI Protein Information: shugoshin-like 1; hSgo1; shugoshin 1AB protein; shugoshin 1CD protein; shugoshin 1EF protein; shugoshin 1GH protein; shugoshin 1KL protein; serologically defined breast cancer antigen NY-BR-85
• UniProt Protein Name: Shugoshin-like 1
• UniProt Gene Name: SGOL1
• UniProt Synonym Gene Names: SGO1; hSgo1
• UniProt Entry Name: SGOL1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the shugoshin family of proteins. This protein is thought to protect centromeric cohesin from cleavage during mitotic prophase by preventing phosphorylation of a cohesin subunit. Reduced expression of this gene leads to the premature loss of centromeric cohesion, mis-segregation of sister chromatids, and mitotic arrest. Evidence suggests that this protein also protects a small subset of cohesin found along the length of the chromosome arms during mitotic prophase. An isoform lacking exon 6 has been shown to play a role in the cohesion of centrioles (PMID: 16582621 and PMID:18331714). Mutations in this gene have been associated with Chronic Atrial and Intestinal Dysrhythmia (CAID) syndrome, characterized by the co-occurrence of Sick Sinus Syndrome (SSS) and Chronic Intestinal Pseudo-obstruction (CIPO) within the first four decades of life (PMID:25282101). Fibroblast cells from CAID patients exhibited both increased cell proliferation and higher rates of senescence. Pseudogenes of this gene have been found on chromosomes 1 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]

Uniprot Description

SGO1: Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity. Belongs to the shugoshin family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 3p24.3

Cellular Component: kinetochore; nucleoplasm; spindle pole; centrosome; cytoplasm; cytosol; condensed nuclear chromosome, pericentric region; chromosome, pericentric region

Molecular Function: protein binding; kinase binding

Biological Process: mitosis; cell division; mitotic cell cycle; attachment of spindle microtubules to kinetochore; meiotic chromosome segregation; centriole-centriole cohesion; chromosome segregation

Disease: Chronic Atrial And Intestinal Dysrhythmia