Human Norrin ELISA Kit | NDP elisa kit

Human Norrin ELISA Kit

Cat. Num. AAA762296

• Gene Names: NDP; ND; EVR2; FEVR
• Reactivity: Human
• Synonyms: Norrin; Human Norrin ELISA Kit; NDP/Norrin/Norrie disease protein/pseudoglioma/X-linked exudative vitreoretinopathy 2 protein/EVR2/exudative vitreoretinopathy 2 (X-linked)/FEVR/ND; NDP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NDP . No significant cross-reactivity or interference between NDP and analogues was observed.
• Sequence Length: 133

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NDP were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NDP were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NDP elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- NDP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- NDP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the NDP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of NDP can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4557789
• NCBI GeneID: 4693
• NCBI Accession #: NP_000257.1
• NCBI GenBank Nucleotide #: NM_000266.3
• Molecular Weight: 15,044 Da
• NCBI Official Full Name: norrin
• NCBI Official Synonym Full Names: Norrie disease (pseudoglioma)
• NCBI Official Symbol: NDP
• NCBI Official Synonym Symbols: ND; EVR2; FEVR
• NCBI Protein Information: norrin
• UniProt Protein Name: Norrin
• Protein Family: Norrin
• UniProt Gene Name: NDP
• UniProt Synonym Gene Names: EVR2
• UniProt Entry Name: NDP_HUMAN

NCBI Description

This gene encodes a secreted protein with a cystein-knot motif that activates the Wnt/beta-catenin pathway. The protein forms disulfide-linked oligomers in the extracellular matrix. Mutations in this gene result in Norrie disease and X-linked exudative vitreoretinopathy. [provided by RefSeq, Feb 2009]

Uniprot Description

NDP: Activates the canonical Wnt signaling pathway through FZD4 and LRP5 coreceptor. Plays a central role in retinal vascularization by acting as a ligand for FZD4 that signals via stabilizing beta-catenin (CTNNB1) and activating LEF/TCF-mediated transcriptional programs. Acts in concert with TSPAN12 to activate FZD4 independently of the Wnt-dependent activation of FZD4, suggesting the existence of a Wnt-independent signaling that also promote accumulation the beta-catenin (CTNNB1). May be involved in a pathway that regulates neural cell differentiation and proliferation. Possible role in neuroectodermal cell-cell interaction. Defects in NDP are the cause of Norrie disease (ND); also known as atrophia bulborum hereditaria or Episkopi blindness. ND is a recessive disorder characterized by very early childhood blindness due to degenerative and proliferative changes of the neuroretina. Approximately 50% of patients show some form of progressive mental disorder, often with psychotic features, and about one-third of patients develop sensorineural deafness in the second decade. In addition, some patients have more complex phenotypes, including growth failure and seizure. Defects in NDP are the cause of vitreoretinopathy exudative type 2 (EVR2). EVR2 is a disorder of the retinal vasculature characterized by an abrupt cessation of growth of peripheral capillaries, leading to an avascular peripheral retina. This may lead to compensatory retinal neovascularization, which is thought to be induced by hypoxia from the initial avascular insult. New vessels are prone to leakage and rupture causing exudates and bleeding, followed by scarring, retinal detachment and blindness. Clinical features can be highly variable, even within the same family. Patients with mild forms of the disease are asymptomatic, and their only disease related abnormality is an arc of avascular retina in the extreme temporal periphery.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: Xp11.4

Cellular Component: cell surface; extracellular matrix; extracellular space

Molecular Function: cytokine activity; frizzled binding; growth factor activity; protein binding; protein homodimerization activity

Biological Process: cell proliferation; cell-cell signaling; nervous system development; placenta development; positive regulation of transcription factor activity; positive regulation of transcription, DNA-dependent; sensory perception of sound; signal transduction; vacuole organization and biogenesis; visual perception; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin

Disease: Exudative Vitreoretinopathy 2, X-linked; Norrie Disease