Human Serglycin (SRGN) ELISA Kit | SRGN elisa kit

Human Serglycin (SRGN) ELISA Kit

Cat. Num. AAA452521

• Gene Names: SRGN; PPG; PRG; PRG1
• Reactivity: Human
• Synonyms: Serglycin (SRGN); Human Serglycin (SRGN) ELISA Kit; PRG; PPG; PRG1; proteoglycan 1; secretory granule; SRGN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SRGN. No significant cross-reactivity or interference between SRGN and analogues was observed.
• Sequence Length: 158

• Samples: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.064ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SRGN were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SRGN were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SRGN elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SRGN in human serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SRGN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SRGN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SRGN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SRGN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1005261256
• NCBI GeneID: 5552
• NCBI Accession #: NP_001307982.1
• NCBI GenBank Nucleotide #: NM_001321053.1
• UniProt Accession #: P10124; Q5VW06; B2R4L7
• Molecular Weight: 17,652 Da
• NCBI Official Full Name: serglycin isoform 1
• NCBI Official Synonym Full Names: serglycin
• NCBI Official Symbol: SRGN
• NCBI Official Synonym Symbols: PPG; PRG; PRG1
• NCBI Protein Information: serglycin
• UniProt Protein Name: Serglycin
• Protein Family: Serglycin
• UniProt Gene Name: SRGN
• UniProt Synonym Gene Names: PRG; PRG1; P.PG

NCBI Description

This gene encodes a protein best known as a hematopoietic cell granule proteoglycan. Proteoglycans stored in the secretory granules of many hematopoietic cells also contain a protease-resistant peptide core, which may be important for neutralizing hydrolytic enzymes. This encoded protein was found to be associated with the macromolecular complex of granzymes and perforin, which may serve as a mediator of granule-mediated apoptosis. Two transcript variants, only one of them protein-coding, have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

Plays a role in formation of mast cell secretory granules and mediates storage of various compounds in secretory vesicles. Required for storage of some proteases in both connective tissue and mucosal mast cells and for storage of granzyme B in T-lymphocytes. Plays a role in localizing neutrophil elastase in azurophil granules of neutrophils. Mediates processing of MMP2. Plays a role in cytotoxic cell granule-mediated apoptosis by forming a complex with granzyme B which is delivered to cells by perforin to induce apoptosis. Regulates the secretion of TNF-alpha and may also regulate protease secretion. Inhibits bone mineralization.