Typical Testing Data/Standard Curve (for reference only)
Human SEMA4C ELISA Kit | SEMA4C elisa kit
Human SEMA4C (Semaphorin 4C) ELISA Kit
Gene Names
SEMA4C; SEMAF; SEMAI; SEMACL1; M-SEMA-F
Reactivity
Human
Synonyms
SEMA4C; Human SEMA4C (Semaphorin 4C) ELISA Kit; SEMA4C elisa kit
Reactivity
Human
Specificity
This kit recognizes Human SEMA4C in samples. No significant cross-reactivity or interference between Human SEMA4C and analogues was observed.
Sequence Length
833
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.16-10ng/mL
Sensitivity
0.1ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human SEMA4C were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human SEMA4C were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SEMA4C elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human SEMA4C concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SEMA4C. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SEMA4C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SEMA4C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human SEMA4C. You can calculate the concentration of Human SEMA4C in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SEMA4C. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SEMA4C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SEMA4C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human SEMA4C. You can calculate the concentration of Human SEMA4C in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
92,623 Da
NCBI Official Full Name
semaphorin-4C
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4C
NCBI Official Symbol
SEMA4C
NCBI Official Synonym Symbols
SEMAF; SEMAI; SEMACL1; M-SEMA-F
NCBI Protein Information
semaphorin-4C; M-Sema F
UniProt Protein Name
Semaphorin-4C
Protein Family
UniProt Gene Name
SEMA4C
UniProt Synonym Gene Names
KIAA1739; SEMAI
UniProt Entry Name
SEM4C_HUMAN
Uniprot Description
SEMA4C: Cell surface receptor for PLXNB2 that plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal brain development, axon guidance and cell migration. Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade. Belongs to the semaphorin family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 2q11.2
Cellular Component: extracellular space; postsynaptic membrane; synaptic vesicle membrane; postsynaptic density; integral to membrane; cell junction
Molecular Function: receptor activity
Biological Process: cell migration in hindbrain; muscle cell differentiation; neural tube closure; cerebellum development; positive regulation of stress-activated MAPK cascade
Research Articles on SEMA4C
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Product Notes
The Human SEMA4C sema4c (Catalog #AAA2504258) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2504258 ELISA Kit recognizes Human SEMA4C. It is sometimes possible for the material contained within the vial of "SEMA4C, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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