Human Secretin (SCT) ELISA Kit | SCT elisa kit

Human Secretin (SCT) ELISA Kit

Cat. Num. AAA2022007

• Reactivity: Human
• Synonyms: Secretin (SCT); Human Secretin (SCT) ELISA Kit; SCT elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Secretin (SCT).; No significant cross-reactivity or interference between Secretin (SCT) and analogues was observed.
• Sequence Length: 121

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 18.52-1,500pg/mL
• Sensitivity: < 7.17pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secretin (SCT) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secretin (SCT) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SCT elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Secretin (SCT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Secretin (SCT) and unlabeled Secretin (SCT) (Standards or samples) with the pre-coated antibody specific to Secretin (SCT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Secretin (SCT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Secretin (SCT) in the sample.

Product Categories/Family for SCT elisa kit

Endocrinology; Hepatology; Hormone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 11345450
• NCBI GeneID: 6343
• NCBI Accession #: NP_068739.1
• NCBI GenBank Nucleotide #: NM_021920.2
• Molecular Weight: 13,016 Da
• NCBI Official Full Name: secretin preproprotein
• NCBI Official Synonym Full Names: secretin
• NCBI Official Symbol: SCT
• NCBI Protein Information: secretin
• UniProt Protein Name: Secretin
• Protein Family: Secretin
• UniProt Gene Name: SCT
• UniProt Entry Name: SECR_HUMAN

NCBI Description

Secretin belongs to the glucagon family. This protein is an endocrine hormone and its major site of production is the endocrine S cells located in the proximal small intestinal mucosa. The release of active secretin is stimulated by either fatty acids or an acidic pH in the duodenum. This hormone stimulates the secretion of bicarbonate-rich pancreatic fluids and has also been shown to regulate the growth and development of the stomach, small intestine, and pancreas. Secretin deficiency has been implicated in autistic syndrome, suggesting that the hormone could have a neuroendocrine function in addition to its role in digestion. [provided by RefSeq, Jul 2008]

Uniprot Description

SCT: Stimulates formation of NaHCO(3)-rich pancreatic juice and secretion of NaHCO(3)-rich bile and inhibits HCl production by the stomach. Belongs to the glucagon family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular region

Molecular Function: hormone activity

Biological Process: dentate gyrus development; visual learning; negative regulation of neuron apoptosis; pancreatic juice secretion