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Typical Testing Data/Standard Curve (for reference only)

Human secreted frizzled-related protein 5 (SFRP5) ELISA Kit | SFRP5 elisa kit

Human secreted frizzled-related protein 5 (SFRP5) ELISA Kit

Gene Names
SFRP5; SARP3
Reactivity
Human
Synonyms
secreted frizzled-related protein 5 (SFRP5); Human secreted frizzled-related protein 5 (SFRP5) ELISA Kit; SFRP5 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Sequence Length
317
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.5ng/ml - 100ng/ml
Sensitivity
0.25ng/ml
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SFRP5 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Secreted Frizzled-Related Protein 5 (also known as SFRP5) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human SFRP5 antibody. SFRP5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human SFRP5 Antibody is added and binds to SFRP5 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SFRP5 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human SFRP5. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35,563 Da
NCBI Official Full Name
secreted frizzled-related protein 5
NCBI Official Synonym Full Names
secreted frizzled related protein 5
NCBI Official Symbol
SFRP5
NCBI Official Synonym Symbols
SARP3
NCBI Protein Information
secreted frizzled-related protein 5
UniProt Protein Name
Secreted frizzled-related protein 5
UniProt Gene Name
SFRP5
UniProt Synonym Gene Names
FRP1B; SARP3; sFRP-5; FRP-1b; SARP-3

NCBI Description

Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas. [provided by RefSeq, Jul 2008]

Uniprot Description

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP5 may be involved in determining the polarity of photoreceptor, and perhaps, other cells in the retina.

Research Articles on SFRP5

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Product Notes

The Human SFRP5 sfrp5 (Catalog #AAA164067) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA164067 ELISA Kit recognizes Human SFRP5. It is sometimes possible for the material contained within the vial of "secreted frizzled-related protein 5 (SFRP5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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