Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Secreted and transmembrane protein 1 (SECTM1) Standard Curve.)
Human Secreted And Transmembrane Protein 1 (SECTM1) ELISA Kit | SECTM1 elisa kit
Human Secreted And Transmembrane Protein 1 (SECTM1) ELISA Kit
Gene Names
SECTM1; K12; SECTM
Reactivity
Human
Synonyms
Secreted And Transmembrane Protein 1 (SECTM1); Human Secreted And Transmembrane Protein 1 (SECTM1) ELISA Kit; SECTM1; K12; K12 protein; type 1a transmembrane protein; SECTM1 elisa kit
Reactivity
Human
Specificity
Human Secreted and transmembrane protein 1 (SECTM1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SECTM1. No significant cross-reactivity or interference between Human SECTM1 and analogues was observed.
Sequence Length
248
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Secreted and transmembrane protein 1 (SECTM1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Secreted and transmembrane protein 1 (SECTM1) Standard Curve.)
Related Product Information for SECTM1 elisa kit
Description: This Human Secreted and transmembrane protein 1 (SECTM1) ELISA Kit employs a two-site sandwich ELISA to quantitate SECTM1 in samples. An antibody specific for SECTM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SECTM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SECTM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SECTM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: SECTM1 encodes a transmembrane and secreted protein with characteristics of a type 1a transmembrane protein. It is found in a perinuclear Golgi-like pattern and thought to be involved in hematopoietic and/or immune system processes. The predicted 248-amino acid K12 protein has the hydropathic characteristics of a type 1a transmembrane protein, which is defined by an extracellular N terminus and a cleaved signal peptide. The authors demonstrated that the K12 protein behaves biochemically as an integral membrane protein. Using Immunofluorescence, they localized K12 to the Golgi. Western blotting showed that the K12 protein migrates as a cluster of bands at 27 kD. In addition, an approximately 20-kD soluble form of K12 is secreted by cells.
Background: SECTM1 encodes a transmembrane and secreted protein with characteristics of a type 1a transmembrane protein. It is found in a perinuclear Golgi-like pattern and thought to be involved in hematopoietic and/or immune system processes. The predicted 248-amino acid K12 protein has the hydropathic characteristics of a type 1a transmembrane protein, which is defined by an extracellular N terminus and a cleaved signal peptide. The authors demonstrated that the K12 protein behaves biochemically as an integral membrane protein. Using Immunofluorescence, they localized K12 to the Golgi. Western blotting showed that the K12 protein migrates as a cluster of bands at 27 kD. In addition, an approximately 20-kD soluble form of K12 is secreted by cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
secreted and transmembrane protein 1
NCBI Official Synonym Full Names
secreted and transmembrane 1
NCBI Official Symbol
SECTM1
NCBI Official Synonym Symbols
K12; SECTM
NCBI Protein Information
secreted and transmembrane protein 1
UniProt Protein Name
Secreted and transmembrane protein 1
Protein Family
UniProt Gene Name
SECTM1
UniProt Synonym Gene Names
K12
NCBI Description
This gene encodes a transmembrane and secreted protein with characteristics of a type 1a transmembrane protein. It is found in a perinuclear Golgi-like pattern and thought to be involved in hematopoietic and/or immune system processes. [provided by RefSeq, Jul 2008]
Uniprot Description
May be involved in thymocyte signaling.
Research Articles on SECTM1
Similar Products
Product Notes
The Human SECTM1 sectm1 (Catalog #AAA9714388) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714388 ELISA Kit recognizes Human SECTM1. It is sometimes possible for the material contained within the vial of "Secreted And Transmembrane Protein 1 (SECTM1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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