Human SCUBE3 (Signal peptide, CUB and EGF-like domain-containing protein 3) ELISA Kit | SCUBE3 elisa kit

Human SCUBE3 (Signal peptide, CUB and EGF-like domain-containing protein 3) ELISA Kit

Cat. Num. AAA7606090

• Gene Names: SCUBE3; CEGF3
• Reactivity: Human
• Synonyms: SCUBE3 (Signal peptide; CUB and EGF-like domain-containing protein 3); Human SCUBE3 (Signal peptide; CUB and EGF-like domain-containing protein 3) ELISA Kit; SCUBE3 elisa kit

• Reactivity: Human
• Sequence Length: 992

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SCUBE3 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- SCUBE3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-SCUBE3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the SCUBE3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of SCUBE3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 734703973
• NCBI GeneID: 222663
• NCBI Accession #: NP_001290065.1
• NCBI GenBank Nucleotide #: NM_001303136.1
• UniProt Accession #: Q8IX30; Q5CZB3; Q86UZ9; Q8NAU9; A8K5A3
• Molecular Weight: 111,099 Da
• NCBI Official Full Name: signal peptide, CUB and EGF-like domain-containing protein 3 isoform 2
• NCBI Official Synonym Full Names: signal peptide, CUB domain and EGF like domain containing 3
• NCBI Official Symbol: SCUBE3
• NCBI Official Synonym Symbols: CEGF3
• NCBI Protein Information: signal peptide, CUB and EGF-like domain-containing protein 3
• UniProt Protein Name: Signal peptide, CUB and EGF-like domain-containing protein 3
• Protein Family: Signal peptide, CUB and EGF-like domain-containing protein
• UniProt Gene Name: SCUBE3

NCBI Description

This gene encodes a member of the signal peptide, complement subcomponents C1r/C1s, Uegf, bone morphogenetic protein-1 and epidermal growth factor-like domain containing protein family. Overexpression of this gene in human embryonic kidney cells results in secretion of a glycosylated form of the protein that forms oligomers and tethers to the cell surface. This gene is upregulated in lung cancer tumor tissue compared to healthy tissue and is associated with loss of the epithelial marker E-cadherin and with increased expression of vimentin, a mesenchymal marker. In addition, the protein encoded by this gene is a transforming growth factor beta receptor ligand, and when secreted by cancer cells, it can be cleaved in vitro to release the N-terminal epidermal growth factor-like repeat domain and the C-terminal complement subcomponents C1r/C1s domain. Both the full length protein and C-terminal fragment can bind to the transforming growth factor beta type II receptor to promote the epithelial-mesenchymal transition and tumor angiogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

SCUBE3: Binds to TGFBR2 and activates TGFB signaling. In lung cancer cells, could serve as an endogenous autocrine and paracrine ligand of TGFBR2, which could regulate TGFBR2 signaling and hence modulate epithelial-mesenchymal transition and cancer progression. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p21.31

Cellular Component: cell surface; extracellular space

Molecular Function: protein binding

Biological Process: cell differentiation; protein heterooligomerization; protein homooligomerization; signal transduction