Human SLC5A8 (Solute carrier family 5 member 8) ELISA Kit | SLC5A8 elisa kit

Human SLC5A8 (Solute carrier family 5 member 8) ELISA Kit

Cat. Num. AAA7608135

• Gene Names: SLC5A8; AIT; SMCT; SMCT1
• Reactivity: Human
• Synonyms: SLC5A8 (Solute carrier family 5 member 8); Human SLC5A8 (Solute carrier family 5 member 8) ELISA Kit; Sodium-coupled monocarboxylate transporter 1; Apical iodide transporter; Electrogenic sodium monocarboxylate cotransporter; Sodium iodide-related cotransporter; Solute carrier family 5 member 8; SLC5A8 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SLC5A8. No significant cross-reactivity or interference between SLC5A8 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SLC5A8 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 296452898
• NCBI GeneID: 160728
• UniProt Accession #: Q8N695; Q2TB99; Q7Z2H9
• Molecular Weight: 110.0
• NCBI Official Full Name: Sodium-coupled monocarboxylate transporter 1
• NCBI Official Synonym Full Names: solute carrier family 5 (sodium/monocarboxylate cotransporter), member 8
• NCBI Official Symbol: SLC5A8
• NCBI Official Synonym Symbols: AIT; SMCT; SMCT1
• NCBI Protein Information: sodium-coupled monocarboxylate transporter 1; apical iodide transporter; solute carrier family 5 member 8; sodium iodide-related cotransporter; electrogenic sodium monocarboxylate cotransporter; solute carrier family 5 (iodide transporter), member 8
• UniProt Protein Name: Sodium-coupled monocarboxylate transporter 1
• Protein Family: Sodium-coupled monocarboxylate transporter
• UniProt Gene Name: SLC5A8
• UniProt Synonym Gene Names: AIT; SMCT; SMCT1
• UniProt Entry Name: SC5A8_HUMAN

NCBI Description

SLC5A8 has been shown to transport iodide by a passive mechanism (Rodriguez et al., 2002 [PubMed 12107270]) and monocarboxylates and short-chain fatty acids by a sodium-coupled mechanism (Gopal et al., 2004 [PubMed 15322102]). In kidney, SLC5A8 functions as a high-affinity sodium-coupled lactate transporter involved in reabsorption of lactate and maintenance of blood lactate levels (Thangaraju et al., 2006 [PubMed 16873376]).[supplied by OMIM, Dec 2008]

Uniprot Description

SLC5A8: Acts as an electrogenic sodium (Na(+)) and chloride (Cl-)-dependent sodium-coupled solute transporter, including transport of monocarboxylates (short-chain fatty acids including L-lactate, D-lactate, pyruvate, acetate, propionate, valerate and butyrate), lactate, mocarboxylate drugs (nicotinate, benzoate, salicylate and 5-aminosalicylate) and ketone bodies (beta-D- hydroxybutyrate, acetoacetate and alpha-ketoisocaproate), with a Na(+):substrate stoichiometry of between 4:1 and 2:1. Catalyzes passive carrier mediated diffusion of iodide. Mediates iodide transport from the thyrocyte into the colloid lumen through the apical membrane. May be responsible for the absorption of D- lactate and monocarboxylate drugs from the intestinal tract. Acts as a tumor suppressor, suppressing colony formation in colon cancer, prostate cancer and glioma cell lines. May play a critical role in the entry of L-lactate and ketone bodies into neurons by a process driven by an electrochemical Na(+) gradient and hence contribute to the maintenance of the energy status and function of neurons. Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.

Protein type: Transporter, SLC family; Transporter; Membrane protein, multi-pass; Membrane protein, integral; Tumor suppressor; Apoptosis

Chromosomal Location of Human Ortholog: 12q23.1

Cellular Component: apical plasma membrane; plasma membrane; integral to membrane

Molecular Function: symporter activity

Biological Process: apoptosis; sodium ion transport; ion transport; transmembrane transport