Human SCF ELISA Kit | SCF elisa kit
SCF (Human) OmniKine ELISA Kit
Rat: SCF
The antigens listed below were tested at 50 ng/ml and exhibited less than 5% cross reactivity.
Murine: SCF
The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
Human: G-CSF, GM-CSF, IL-1alpha, IL-1beta, IL-2, M-CSF, PDGF-AA, PDGF-AB, PDGF-BB
Murine: G-CSF, GM-CSF, M-CSF
Rat: GM-CSF
Testing Data
(The OmniKine Human SCF ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human SCF proteins within the range of 16-2000 pg/ml.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
SCF: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5- trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. Homodimer, non-covalently linked (Probable). Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation. Belongs to the SCF family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 12q21.32
Cellular Component: cytoplasm; cytoskeleton; extracellular region; extracellular space; filopodium; integral component of membrane; lamellipodium; plasma membrane
Molecular Function: cytokine activity; growth factor activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; Ras guanyl-nucleotide exchange factor activity; stem cell factor receptor binding
Biological Process: cell adhesion; cell proliferation; embryonic hemopoiesis; germ cell programmed cell death; male gonad development; MAPK cascade; negative regulation of mast cell apoptosis; neural crest cell migration; ovarian follicle development; phosphatidylinositol phosphorylation; phosphoinositide-mediated signaling; positive regulation of DNA replication; positive regulation of GTPase activity; positive regulation of leukocyte migration; positive regulation of MAP kinase activity; positive regulation of melanocyte differentiation; positive regulation of myeloid leukocyte differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of Ras protein signal transduction; regulation of phosphatidylinositol 3-kinase signaling; signal transduction
Disease: Deafness, Autosomal Dominant 69; Hyperpigmentation, Familial Progressive, 2; Skin/hair/eye Pigmentation, Variation In, 7
